MRI、CT联合ELISA法检测血清AFP-L3含量在原发性肝癌诊断中的价值

The clinical value of the combined detection of alpha- fetoprotein- L3 detected by enzyme linked immunosorbent assay and MRI,contrast- enhanced CT in the diagnosis of primary hepatic cancer

目的:评价MRI、CT扫描联合酶联免疫吸附法(ELISA)检测血清甲胎蛋白异质体(AFP-L3)含量在原发性肝癌(PHC)诊断中的价值。方法:137例血清标本来自唐山市人民医院2011年3月至11月间的住院患者,运用ELISA法检测血清中AFP-L3的含量,以AFP-L3>37.89ng/ml为临界值。137例病例均具有CT、MRI资料,回顾分析探讨采用血清AFP-L3含量与CT、MRI及这三种方法联合应用在PHC诊断方面的价值。结果:以AFP-L3>37.89ng/ml为临界值诊断PHC的灵敏度为71.73%、特异度为88.88%。联合运用血清AFP-L3含量以及CT扫描两种技术诊断PHC的灵敏度为85.87%、特异度为93.33%。三种方法联合应用诊断PHC的灵敏度为89.13%、特异度为93.67%。结论:联合运用ELISA法检测AFP-L3含量及CT、MRI三种技术对PHC诊断具有重要的价值,在对血清AFP-L3含量低的PHC患者诊断中行CT、MRI扫描具有非常重要的意义。

Objective： The clinical value of the combined detection of alpha - fetoprotein - L3 detected by enzyme linked immunosorbent assay and MRI, contrast - enhanced CT in the diagnosis of primary hepatic cancer was evalua- ted. Methods：The serum samples of 137 patients with positive AFP treated in our hospital between March and No- vember 2011 were studied. Using ELISA to detect the levels of serum AFP- L3 ,AFP- L3 level 〉 37.89ng/ml as di- agnostic criteria. All 137 cases were examined with MRI, CT scans ,to explore the content of AFP- L3 in serum and CT enhancement scanning and the combination of the two methods in the diagnosis of PHC value. Results：Taking AFP- L3 level 〉 37.89ng/ml as diagnostic criteria, the sensitivity of AFP- L3 level in PHC diagnosis was 71.73% and the specificity was 88.88%. The sensitivity of AFP - L3 level and CT scans of two kinds of technique in the diagnosis of PHC was 85.87% ,the specificity was 93.33%. Using three kinds of techniques in the diagnosis of PHC sen- sitivity was 89.13 %, specificity was 93.67%. Conclusion： ELISA has a high clinical value in detecting AFP - L3 which is a valuable biomarker, AFP - L3 level and MRI, CT scan of two kinds of techniques have important value in the diagnosis and prediction of prognosis of PHC.