肝胆疾病生物标本库的建立

Establishment of tumor bank of hepatic and biliary tract diseases

目的:建立肝胆系统疾病血液及组织标本库,为肝胆各种疾病在分子水平的研究提供基础。方法:选取2013年7月至2014年4月我院肝胆外科以原发性肝癌、胆囊癌及胆囊结石为主要诊断而行手术切除的患者标本,留取血浆、血清及组织标本,制作石蜡包块并行常规病理组织学鉴定,并定期使用Trizol试剂提取总RNA及基因组DNA以进行标本质量控制。结果:以原发性肝癌为主要诊断者血液标本388例,组织标本67例,以胆囊癌为主要诊断者血液及组织标本78例,术后均进行病理诊断,其中胆囊癌47例,胆管癌31例。此外,本研究也收集胆结石患者的血液标本1154例。结论:本实验建立的肝胆疾病标本采集流程有效可行,临床资料收集完备,能够为今后肝胆肿瘤的发生发展机制研究提供较好的标本资源。

Objective：To explore a rational and effective procedure to establish a tissue and plasma sample bank for hepatic and biliary tract diseases. Methods：Plasma, serum and tissue samples from patients who were diagnosed as biliary tract tumors, primary hepatic carcinoma or gallstone during July 2013 and April 2014 in our hospital were har- vested and stored at - 80~C. Pathological diagnosis was made on paraffin embedded samples. A part of the tissues was used to isolate total RNA by using Trizol reagent and extract cDNA. Results：There were 388 blood samples and 67 excised samples diagnosed as having HCC. 78 blood sample and excised samples diagnosed as having biliary tract sys- tem tumors included 47 gallbladder carcinoma, 31 cholangiocarcinoma. In addition, 1154 blood samples of gallstone were collected. Conclusion ： The present program for establishment of samples bank is feasible and provide sample re- sources for hepatic and biliary tract carcinoma research.