钛表面微沟槽形貌对人牙龈成纤维细胞接触诱导效应的比较研究

Comparative study on contact guidance activity of human gingival fibroblasts on microgroove surfaces

目的 比较钛表面不同尺寸微沟槽对人牙龈成纤维细胞（human gingival fibroblast,HGF）接触诱导效应的差异,以期为种植体穿龈部分微沟槽尺寸设计提供依据.方法设计微沟槽宽度与间距相等,利用光刻技术在硅片表面制作宽度分别为15、30、60 μm,深度分别为5和10 μm的微沟槽,分别命名为T15/5、T15/10、T30/5 、T30/10、T60/5、T60/10组,各组硅片表面溅射钛;以溅射钛的硅片为对照组（每组样本量为7）.免疫荧光观察细胞沿沟槽排列的形态,通过细胞核排列一致性比例及细胞核形变率进行定量比较.结果 细胞核排列一致性比例及细胞核形变率各微沟槽组均大于对照组,细胞核排列一致性比例T60/10组（0.937±0.024）最高,T15/5组（0.660±0.016）最低.细胞核形变率T60/10组（3.555±0.205）最高,T15/5组（1.819±0.011）最低.结论 不同尺寸微沟槽均可对HGF起接触诱导作用,程度随沟槽宽度和深度的增加而增大.

Objective To compared the difference incontact guidance activity among microgroove surfaces with different sizes of human gingival fibroblast（HGF）,with the hope of providing basis for size selection of microgroove for transmucosal part of dental implant.Methods Basing on the size of HGF,microgroove titanium surfaces were fabricated by photolithography with parallel grooves：15,30 or 60 μm in width and 5 or 10 μm in depth.The groups that used different microgroove surfaces were denoted as T15/5,T15/10,T30/5,T30/10,T60/5,and T60/10.Group T0（the control meanwhile was a sputter of titanium on a simple planar silicon substrate）.The morphology that HGF arranged along the groove was analyzed by immunofluorescence staining.Difference in contact guidance activity was quantitatively compared basing on the consistency of nucleus arrangement and deformation ratio.Results Microgroove groups had significantly higher consistency of nucleus arrangement and deformation ratio compared to the control group,with T60/10 had the highest consistency of 0.937±0.024,and T15/5 had the lowest consistency of 0.660± 0.016 and T60/10 had the highest deformation ratio of 3.555±0.205,and T15/5 had the lowest deformation ratio of 1.819±0.011.Conclusions Microgroove surfaces of all the different sizes show contact guidance activity on HGF,and the contact guidance activity increases with the increase of width and depth.