摘要
从燃煤电厂生物滴滤系统填料的生物膜上筛选分离出一株高效好氧反硝化细菌JH8。经生理生化鉴定和16S r DNA序列分析,鉴定该菌为地衣芽孢杆菌(Bacillus licheniformis)。对菌株JH8进行反硝化特性研究,发现该菌可以分别以硝酸盐和亚硝酸盐为氮源,在50℃条件下进行好氧反硝化,24 h内的脱氮率高达86.96%和97.63%。通过单因素实验得到该菌最优脱氮条件:以柠檬酸钠为碳源、转速为160 r/min、C/N为16,优化后该菌24 h内脱氮率可达99%。该菌的分离对于研究高温环境下的好氧反硝化机理具有重要意义。
Baterium JH8,with high nitrogen removal efficiency,was newly isolated from the biofilm of a biofilter which used to remove NOxfrom flue gas. The isolate was identified as Bacillus licheniformis by biochemical studies and 16 S r DNA gene sequencing analysis. It could use both nitrate and nitrite as nitrogen sources,and the removal efficiencies achieved86.96% and 97.63% respectively at 50 ℃ in 24 h. Optimal conditions for the cell growth and denitrification included sodium citrate as carbon source,C/N of 16 and shaking speed of 160 r/min. The isolation of this bacterium is of great significance to the research of aerobic denitrification mechanism under thermophilic conditions.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2015年第1期6-10,67,共6页
Environmental Science & Technology
基金
国家自然科学基金(51378217)
广东自然科学基金(S2012020010887)
关键词
好氧反硝化
高温
地衣芽孢杆菌
单因素实验
aerobic denitrification
thermophilic
Bacillus licheniformis
single-factor experiment
