摘要
目的了解褪色沙雷菌临床分离株的医院感染现状,建立随机扩增多态性DNA(RAPD)基因分型方法,对褪色沙雷菌临床分离株进行基因分型,为控制医院感染提供分子流行病学资料。方法收集2010年1月-2012年12月临床送检的各类感染性标本,使用常规方法进行分离培养,采用VITEK-2Compact全自动微生物分析系统进行菌种鉴定,并对临床常用抗菌药物进行MIC测定,对临床分离的287株褪色沙雷菌的标本来源、临床分布及其耐药谱采用WHONET5.6软件进行统计分析,采用RAPD技术进行基因分型。结果 287株褪色沙雷菌主要来自于ICU、呼吸科和神经内科,分别占37.6%、31.7%和14.6%;标本来源依次为呼吸道、泌尿道和手术部位,分别占77.7%、11.1%和8.0%;褪色沙雷菌对美罗培南、头孢哌酮/舒巴坦和亚胺培南的耐药率<10.0%,对氨苄西林、哌拉西林和头孢曲松等耐药率均>70.0%,RAPD技术将287株褪色沙雷菌分为12个基因型,以A、D型为主。结论褪色沙雷菌主要来自于ICU、呼吸科和神经内科病房的呼吸道标本,其多药耐药现象较为严重,本地区褪色沙雷菌可分为12个基因型,以A、D型最为常见。
OBJECTIVE To understand the current situation of hospital infection of regional clinical isolates of Serratia rnarcescens, to establish random amplified polymorphic DNA (RAPD) genotyping methods for genotyping of S. marcescens clinical isolates to provide molecular epidemiological data for the control of nosocomial infections. METHODS Infectious clinical specimens were collected between Jan. 2010 - Dec. 2012, and were isolated and cultured using conventional methods. VITEK-2 Compact automatic microbial analysis system was used for strain identification and MIC determination was conducted to commonly used antimicrobial drugs. WHO- NET5.6 software was used to statistically analyze the specimen sources, clinical distribution and drug resistance spectrum of the 287 clinical isolates of S. marcescens. Genotyping was performed by RAPD technique. RESULTS The 287 strains of S. rnarcescens were mainly from ICU, respiratory and neurology ward, accounting for 37.6 %, 31.7M and 14. 6%, respectively. The sources of specimens included respiratory tract 77. 7%, urinary tract 11.1M and operative site 8.0%. Drug sensitivity results showed, the drug resistance rates of S. rnarcescens to meropenem and imipenem, sulperazon were below 10. 0M, while the rates to ampicillin, piperacillin and ceftriaxone were greater than 70.0%. Using RAPD technique, the 287 strains of S. marcescens were divided into 12 genotypes, A--L type , in which A and D were the dominant types. CONCLUSION S. rnarcescens was mainly from the respiratory specimens of ICU, respiratory and neurology ward, and the multidrug resistance phenomenon was quite serious. S. marcescens in the region could be divided into 12 genotypes, with A and D as the most common types.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第3期492-494,共3页
Chinese Journal of Nosocomiology
基金
山东省科技厅基金资助项目(2009GG0002057)
青岛市公共领域科技支撑计划项目(2012-1-3-1-[18]-nsh)
关键词
褪色沙雷菌
医院感染
耐药性
基因分型
Serratia marcescens
Nosocomial infections
Resistance
Genotyping