沉香药材HPLC-DAD特征图谱研究

HPLC-DAD Characteristic Chromatogram of Chinese Eaglewood

目的建立沉香药材的HPLC-DAD特征图谱。方法采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),检测波长252 nm,柱温30℃,流动相为乙腈-0.1%甲酸水溶液,梯度洗脱,流速0.7 m L·min-1,对23个批次沉香药材进行分析,并利用《中药色谱指纹图谱相似度评价系统》2004A进行数据处理。结果通过多点校正及自动匹配,共确定了17个共有峰。通过与标准品比对,确证了其中的12个色谱峰。综合各批次沉香样品的差异、各成分含量及分离度,将其中的9个色谱峰设定为沉香药材的特征峰。以色谱峰1(沉香四醇)为参照,其他8个色谱峰的相对保留时间分别为1.033 6、1.219 3、1.248 6、1.783 3、1.891 5、1.918 4、2.404 6和2.458 2。结论该方法符合方法学验证要求,可用于沉香药材的质量控制。

OBJECTIVE To establish the characteristic chromatogram of Chinese eaglewood by HPLC-DAD. METHODS The analysis was performed on a Diamonsil C18 column （4. 6 mmx 250 mm, 5 μm） with a gradient mobile phase of acetonitrile-0. 1% formic acid at a flow rate of 0. 7 mL · min^ -1. The detection wavelength was set at 252 nm and the column temperature was 30℃. Twenty- three batches of Chinese eaglewood samples were analyzed with the developed method, and similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine（2004A Version） was used for the quality assessment. RESULTS Seventeen common peaks were found through the similarity evaluation system and 12 peaks were confirmed by comparison with the chromatograms of the standard substances. Among them, 9 peaks were identified as characteristic peaks of Chinese eaglewood. With peak 1 （agarotetrol） as the reference, the relative retention time of the other eight peaks were 1. 033 6, 1. 219 3, 1. 248 6, 1. 783 3, 1. 891 5, 1. 918 4, 2. 404 6 and 2. 458 2, respectively. CONCLUSION The developed method conforms to the requirements of methodology validation, and can be used for the quality control of Chinese eaglewood.