根皮素对rBMECs摄取人参皂苷Rb1的影响

The Effect of Phloretin on rBMECs Uptake of Ginsenoside Rb1

目的以原代培养的大鼠脑微血管内皮细胞为模型,研究根皮素对大鼠脑微血管内皮细胞摄取人参皂苷Rb1的影响。方法实验分为2组,人参皂苷Rb1对照组和根皮素干预组。运用LC-MS/MS测定大鼠脑微血管内皮细胞对人参皂苷Rb1的摄取量,BCA法测细胞蛋白浓度。结果根皮素干预组大鼠脑微血管内皮细胞对人参皂苷Rb1的摄取量显著低于人参皂苷Rb1对照组(P<0.05)。结论根皮素可显著抑制大鼠脑微血管内皮细胞对人参皂苷Rb1的摄取。

OBJECTIVE To evaluate the effect of phloretin on the uptake of ginsenoside Rbl by the cell model, primary rat brain microvascular endothelial cells, （rBMEC） in vitro. METHODS Experiments were divided into two groups, ginsenoside Rbl with and without the addition of phloretin intervention. The cellular uptake of ginsenoside Rbl was determined by LC-MS/ MS. Protein content in cultured cells was measured by the method of BCA. RESULTS The uptake amount of ginsenoside Rbl in rBMEC with phloretin was significantly lower（ P 〈 0.05 ）. CONCLUSION The uptake of ginsenoside Rbl by rBMEC is significantly inhibited by phloretin.