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低剂量γ-射线照射人淋巴母细胞诱导BTG2基因mRNA变化

Changes of BTG2 gene m RNA expression induced by low-dose γ-rays in human lymphoblastoid cells
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摘要 为观察低剂量γ-射线对人淋巴母细胞AHH1 B细胞转位基因2(B cell translocation gene 2,BTG2)基因m RNA表达水平的影响,探讨BTG2基因作为低剂量范围辐射生物剂量计的可能性,采用0-1.0 Gyγ-射线照射人淋巴母细胞,照射后不同时间点(0-72 h)提取细胞总RNA,采用实时荧光定量PCR方法检测BTG2基因的表达变化,分析该基因的时间-剂量效应关系。结果表明,照射后BTG2基因转录水平表达在低剂量范围呈剂量依赖增加,照射后24 h达峰值,之后逐渐下降,在48 h和72 h时处于平台期,在168 h恢复至初始水平。BTG2基因对低剂量电离辐射敏感,有较好的剂量效应和时间效应关系,具有开发为低剂量辐射生物剂量计的潜力。 The aim is to investigate the effects of low-dose γ-rays on gene expression of BTG2 and the probability of it as bio-dosimeter. AHH-1 lines were irradiated withγ-rays of 0-1.0 Gy. Total RNA of cells was extracted at 0-168 h after irradiation, and real-time quantitative PCR was used to detect BTG2 gene expression. The relationships between the dose or time and gene expression were analyzed. After irradiation, the BTG2 gene expression increased in a dose-dependent form. Its peak expression was at 24 h after irradiation, and then the BTG2 gene expression gradually decreased to platform stage at 48 h and 72 h, and finally returned to the level of 0 h at 168 h. BTG2 gene is sensitive to irradiation and has potential in usage as biological dosimeter for its good dose and time-effect relationships.
作者 何颖 沈先荣 钱甜甜 王庆蓉 蒋定文 陈伟
机构地区 海军医学研究所
出处 《辐射研究与辐射工艺学报》 CAS CSCD 2015年第1期20-24,共5页 Journal of Radiation Research and Radiation Processing
基金 全军计划科研项目(AHJ09J012)资助
关键词 低剂量电离辐射 人淋巴母细胞 BTG2 基因表达 实时荧光定量PCR Low-dose ionizing radiation, Human lymphoblastoid cells, BTG2, Gene expression, Real-timequantitative PCR
分类号 R811.5 [医药卫生—放射医学] TL72 [核科学技术—辐射防护及环境保护]
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辐射研究与辐射工艺学报
2015年 第1期

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