响应面法优化蜂粮引子发酵液的制备条件

Optimization of Preparation Condition Bee Bread Primers Fermentation Broth by Response Surface Methodology

为确定成熟蜂粮引子发酵液的最佳发酵条件,利用中心组合(Box-Behnken)试验设计原理,采用3因素3水平响应面分析法,获得多元二次回归线性方程,以蜂花粉破壁率为响应值作响应面和等高线图。分析结果表明:蜂粮引子发酵液的最佳制备条件为发酵时间15 h、发酵温度35℃、蜂蜜加入量3.0%,在此条件下蜂花粉完全破壁率为53.53%与理论预测值53.944%,相对误差为0.414%,拟合度比较好。

To determine the optimal fermentation conditions mature of bee bread fermentation broth, using central composite experimental design （Box-Behnken）, using three factors and three levels of response surface analysis, to obtain a multiple quadratic regression linear equation, bee pollen wall broken rate as response values for the response surface and contour plot. The analysis results show that the optimal conditions for preparation of bee bread primers fermentation broth were determined follows： honey dosage 3.0% fermentation time 15 h, fermentation temperature 35 ℃, Under such conditions, the relative error between the experimental （53.53%） and theoretical （53.944%） wall broken rates of bee pollen was 6.7%, indicating that the goodness of fit of the established regression model is quiet good.