鱼皮胶原寡肽多级膜分离纯化工艺的优化

The Optimization of Integrated-membrane Separation and Purification Process for Collagen Oligopeptide from Fish Skin

采用多级膜对鱼皮胶原寡肽提取液进行分离。研究了不同截留分子量的超滤膜对提取液分离效果及回收率的影响。结果表明,最适用膜为截留分子量为5 ku的超滤膜,透过液中胶原寡肽占总肽量的96%,胶原寡肽的回收率为92.5%。经超滤膜处理后的料液再用纳滤膜进行脱盐、浓缩。200 u的纳滤膜能够有效地除去鱼皮胶原寡肽提取液中的无机盐等小分子杂质,经过脱盐后的浓缩液中胶原寡肽的分子量集中分布在100-1 000 u之间,料液的电导率从初始的10 ms/cm,下降至86μs/cm。

Integrated membranes were applied for the separation of fish skin collagen oligopeptide. The impact of ultrafiltration membranes with different molecular weight cut-off （MWCO） on separation effect and recovery rate was researched. The result showed that the optimal membrane was the membrane with MWCO 5 ku. In permeate, the ratio of collagen oligopeptide to the total amount of peptide was 96% and the recovery rate of collagen oligopeptide was 92.5%. After ultrafiltration, the extract was desalted and concentrated by nanofiltration. The impurity such as salt could be removed effectively by the nanofiltration membrane with MWCO 200 u. After desalination, molecular weight distribution of collagen peptides in concentrate ranged from 100 to 1 000 u and the conductivity decreased from 10 ms/cm to 86 ps/cm.