摘要
采用多级膜对鱼皮胶原寡肽提取液进行分离。研究了不同截留分子量的超滤膜对提取液分离效果及回收率的影响。结果表明,最适用膜为截留分子量为5 ku的超滤膜,透过液中胶原寡肽占总肽量的96%,胶原寡肽的回收率为92.5%。经超滤膜处理后的料液再用纳滤膜进行脱盐、浓缩。200 u的纳滤膜能够有效地除去鱼皮胶原寡肽提取液中的无机盐等小分子杂质,经过脱盐后的浓缩液中胶原寡肽的分子量集中分布在100-1 000 u之间,料液的电导率从初始的10 ms/cm,下降至86μs/cm。
Integrated membranes were applied for the separation of fish skin collagen oligopeptide. The impact of ultrafiltration membranes with different molecular weight cut-off (MWCO) on separation effect and recovery rate was researched. The result showed that the optimal membrane was the membrane with MWCO 5 ku. In permeate, the ratio of collagen oligopeptide to the total amount of peptide was 96% and the recovery rate of collagen oligopeptide was 92.5%. After ultrafiltration, the extract was desalted and concentrated by nanofiltration. The impurity such as salt could be removed effectively by the nanofiltration membrane with MWCO 200 u. After desalination, molecular weight distribution of collagen peptides in concentrate ranged from 100 to 1 000 u and the conductivity decreased from 10 ms/cm to 86 ps/cm.
出处
《食品工业》
北大核心
2015年第2期174-177,共4页
The Food Industry
基金
国家海洋局第三海洋研究所基本科研业务费专项资金项目(海三科2013029)
厦门海洋研究开发院共建项目(2014)
海洋公益性行业科研专项(201205022-8)
海洋生物产业化中试技术研发公共服务平台(12PZP001SF10)
关键词
膜分离
超滤
纳滤
鱼皮
胶原寡肽
membrane separation
ultrafiltration
nanofiltration
fish skin
collagen oligopeptide