低浓度吉西他滨对肾癌786-0细胞免疫原性相关分子表达的影响

Effects of low dose gemcitabine on the expressions of immunogenic molecular related molecules on the surface of renal carcinoma cell 786-0

目的:探讨低浓度吉西他滨(gemcitabine,GMC)对人肾透明细胞癌786-0细胞免疫原性相关钙网蛋白(calreticulin,CRT)、MHC-Ⅰ/Ⅱ表达变化的影响。方法:依据药物浓度分为4组,用流式细胞仪检测各组细胞CRT、MHC-Ⅰ、MHC-Ⅱ分子的表达。结果:低浓度GMC可使CRT、MHC-Ⅰ/Ⅱ荧光强度表达增强,与对照组比较,低浓度各组CRT、MHC-Ⅰ/Ⅱ表达量差异有统计学意义,组间比较差异均有统计学意义(P<0.01)。结论:低浓度GMC能够有效诱导人肾透明细胞癌免疫原性相关CRT、MHC-Ⅰ/Ⅱ的表达。

Objective To investigate the effect of low dose gemcitabine （GMC） on the expression of calreticulin （CRT） and MHC- Ⅰ/Ⅱ in the renal carcinoma cell 756-0. Methods Four groups were set up based on the gradient of drug concentration. The expressions of CRT and MHC-Ⅰ/Ⅱ were detected by the flow cytometry. Results Expression of CRT and MHC-Ⅰ/Ⅱ in the cell significantly increased in the low dose GMC groups than those in the control group（P 〈 0.01）. Conclusion The low dose of GMC may effectively induce the expression of CRT and MHC-Ⅰ/Ⅱ in the renal clear cell.