HMGA2、Ki-67和MMP-9在恶性星形细胞瘤中的表达及其相关性

Expression of HMGA2, Ki-67 and MMP-9 in malignant astrocytic tumors and their correlation with tumor cell invasion

目的:探讨HMGA2、Ki-67和MMP-9在恶性星形细胞瘤中的表达及其与肿瘤细胞侵袭性的关系。方法:选取2010年3月至2013年4月在我院神经外科手术切除且保存完整的原发性脑星形细胞瘤标本86例,同时选取10例正常脑组织作为对照,采用免疫组化SP法对组织中HMGA2、Ki-67和MMP-9蛋白表达情况进行检测,利用RT-PCR定量检测HMGA2、Ki-67和MMP-9基因m RNA表达情况。结果:HMGA2、Ki-67和MMP-9蛋白均随病理级别增加阳性表达率增加,病理级别越高,阳性表达率越高,差异均具有统计学意义(P<0.05);Spearman相关分析显示,HMGA2蛋白阳性表达与Ki-67和MMP-9表达均呈正相关(r1=0.397,r2=0.463,P<0.05),与星形细胞瘤病理级别呈正相关(P<0.01);HMGA2、Ki-67和MMP-9基因在正常脑组织和不同病理级别星形细胞瘤中表达水平比较差异均具有统计学意义(P<0.05),经LSD-t组间两两比较,各组之间差异均具有统计学意义(P<0.05)。结论:MGA2、Ki-67和MMP-9基因与星形细胞瘤恶性程度和细胞侵袭力密切相关,可以作为判定患者预后的敏感指标。

Objective To investigate the expression of HMGA2, Ki-67 and MMP-9 in malignant astrocytie tumors and their correlation with tumor cell invasion. Methods Eighty-six eases of complete primary brain astrocytoma specimens from surgical resection from March 2010 to April 2013 in the department of Neurosurgery at the Fifth Affiliated Hospital of Zhengzhou University were selected. HMGA2, Ki-67 and MMP-9 protein expression were detected by immunohistochemical SP method. HMGA2, Ki-67 and MMP-9 mRNA gene expressions were detected by RT-PCR quantitative. Results HMGA2, Ki-67 and MMP-9 protein positive expression rates increased when the athological rade ncreased and the differences were all statistically significant （P 〈 0.05）. Spearman correlation analysis showed that, HMGA2 protein expression, Ki-67 and MMP-9 expression were positively correlated （r1 = 0.397, r2 = 0.463, P 〈 0.05）, and was positively correlated with tumor pathological grade （P 〈 0.01）. The differences of HMGA2, Ki-67 and MMP-9 gene expression levels in normal brain tissue and different pathological grade astrocytomas were statistically significant （P 〈 0.05）. LSD-t test between any two groups indicated intergroup statistical significance （P 〈 0.05）. Conclusions HMGA2, Ki- 67 and MMP-9 genes were closely related with the malignancy and invasiveness of astrocytic tumors and they could be used as sensitive indicators of deter mining the prognosis of patients.