浊点萃取法结合超声波提取连翘叶中叶绿素

Extraction of Chlorophyll from Forsythia suspensa Leaves Using Cloud Point Extraction Coupled with Ultrasonication

本实验以连翘叶为材料,首次对浊点萃取法结合超声波提取叶绿素进行了研究。首先采用单因素考察及正交试验筛选,以665 nm处的吸光度值作为考察指标,得叶绿素最佳提取条件是:Triton X-114浓度为4%,超声时间为11 min,料液比为1∶10;其次对该提取方法与传统提取方法进行比较,得叶绿素的提取率比传统有机溶剂提取法提高了9.92%,与超声波提取法相比,提取率提高了11.58%。本研究为叶绿素的提取提供了一种新的方法,该方法提取温度低(室温)、提取过程不使用挥发或有毒溶剂,具有经济、安全、高效、简便、省时、环保等优点,且适用于任何绿色植物茎和叶中叶绿素的提取。

Extraction of chlorophyll from Forsythia suspensa leaves using cloud point extraction coupled with ultrasoniea- tion was investigated in the present study. Single factor test and orthogonal design were used to determine the optimal conditions with absorbance values of 665 rim as the index of evaluation. The optimal conditions for the extraction of chlo- rophyll were as follows：Triton X-114 concentration of 4%, ultrasonic power of 250 W, ultrasonic time of 11 rain, and sol- id-liquid ratio of 1： 10. Comparing the developed extraction method with the traditional organic solvent extraction methods and ultrasonic extraction method,the extraction rate improved 9.92% and 11.58% ,respectively. This study provided a new method for the extraction of cMorophyll with the following advantages ：low temperature （ room temperature） of ex- traction, no using volatile or toxic organic solvents, economical, safe, efficient, convenient time-saving, environmental pro- tection, etc. In addition, the method can be applied for the extraction of chlorophyll from other natural resources.