UPLC-MS同时测定赤芍中五个指标成分的含量

Simultaneous Determination of Five Main Index Compounds in Radix Paeoniae Rubra by UPLC-MS

建立超高效液相色谱-串联质谱法同时测定赤芍药材中的没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷含量的分析方法。采用Acquity UPLC BEH C18柱（2.1 mm×50 mm,1.7μm）;流动相为0.1%甲酸乙腈溶液（A）-0.1%甲酸水溶液（B）,梯度洗脱,电喷雾离子源（ESI）,选择性离子监测（SIR）模式进行正负离子同步监测。没食子酸、氧化芍药苷、芍药内酯苷、芍药苷和苯甲酰芍药苷在0.134~31.250、0.014~2.784、0.471~30.750、0.293~75.040和0.158~40.360μg/m L浓度范围线性关系良好,平均加样回收率为91.36%~112.83%,RSD为1.1%~8.9%。该方法准确、高效、重现性好、专属性高,可用于赤芍药材的质量控制。

To establish a UPLC-MS method for simultaneous determination of gallic acid, oxypaconiilorin, albiflorin, poe- onitlorin and benzoylpaconiilofin in Radix Paeoniae Rubra. An Acquity UPLC BEH Clscolunm （2.1 mm ×50 mm,1.7 μm） was used for the chromatographic separation with the mobile phase consisting of 0. 1% formic acid in acetonitrile （A） and 0.1% formic acid in water （B） by gradient elution. Selected ion recording （SIR） mode was used for detec- tion. The linear ranges of gallic acid, oxypaconitlorin, albitlorin, paconitlorin and benzoylpaconiflorin were determined to be 0. 134 ~ 31. 250,0.014 ~ 2.784,0.471 ~ 30.750,0.293 ~ 75. 040 and 0. 158 ~ 40. 360 ttg/mL, respectively. The average recoveries were 91.36% ~ 112.83% ,with RSDs of 1.1% ~ 8.9%. The developed UPLC-MS method was accu- rate and effective. It can be used for the quality evaluation of Radix Paconiac Rubra with simultaneous determination of 5 marker compounds.