摘要
建立超高效液相色谱-串联质谱法同时测定赤芍药材中的没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、苯甲酰芍药苷含量的分析方法。采用Acquity UPLC BEH C18柱(2.1 mm×50 mm,1.7μm);流动相为0.1%甲酸乙腈溶液(A)-0.1%甲酸水溶液(B),梯度洗脱,电喷雾离子源(ESI),选择性离子监测(SIR)模式进行正负离子同步监测。没食子酸、氧化芍药苷、芍药内酯苷、芍药苷和苯甲酰芍药苷在0.134~31.250、0.014~2.784、0.471~30.750、0.293~75.040和0.158~40.360μg/m L浓度范围线性关系良好,平均加样回收率为91.36%~112.83%,RSD为1.1%~8.9%。该方法准确、高效、重现性好、专属性高,可用于赤芍药材的质量控制。
To establish a UPLC-MS method for simultaneous determination of gallic acid, oxypaconiilorin, albiflorin, poe- onitlorin and benzoylpaconiilofin in Radix Paeoniae Rubra. An Acquity UPLC BEH Clscolunm (2.1 mm ×50 mm,1.7 μm) was used for the chromatographic separation with the mobile phase consisting of 0. 1% formic acid in acetonitrile (A) and 0.1% formic acid in water (B) by gradient elution. Selected ion recording (SIR) mode was used for detec- tion. The linear ranges of gallic acid, oxypaconitlorin, albitlorin, paconitlorin and benzoylpaconiflorin were determined to be 0. 134 ~ 31. 250,0.014 ~ 2.784,0.471 ~ 30.750,0.293 ~ 75. 040 and 0. 158 ~ 40. 360 ttg/mL, respectively. The average recoveries were 91.36% ~ 112.83% ,with RSDs of 1.1% ~ 8.9%. The developed UPLC-MS method was accu- rate and effective. It can be used for the quality evaluation of Radix Paconiac Rubra with simultaneous determination of 5 marker compounds.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第2期282-285,270,共5页
Natural Product Research and Development
基金
贵州省中药现代化科技产业研究开发专项(2013-5062
2013-4001)
贵州省高等学校创新能力提升计划(2013-04)
贵州省科学技术基金(2013-2035)