UPLC-TOF-MS快速测定不同核用银杏品种银杏叶中银杏内酯和白果内酯含量

Determination of Gingkolides and Bibobalide in Ginkgo( Ginkgo biloba) Leaf from Different Species by UPLC-TOF-MS

为比较鄂西南不同核用银杏品种叶片中银杏内酯和白果内酯含量,采用超高压液相色谱-飞行时间质谱（UPLC-TOF-MS）联用方法测定微量银杏内酯和白果内酯含量,分析柱为ZORBAX C18（2.1×50 mm,1.8μm）色谱柱,流动相为乙腈-0.1%甲酸水溶液,采用梯度洗脱,飞行时间质谱作为检测器,选择离子采集方式。方法测定银杏内酯和白果内酯的线性范围为0.2~4μg/m L,相关系数为0.9996以上,相对标准偏差小于1.65%。测定结果表明,在四个核用银杏品种中,恩银23号具有较高的银杏内酯和白果内酯含量,同时白果内酯和银杏内酯A、B及C总含量比例较佳,可以作为叶用银杏发展的优选品种。

In this study, UPLC-TOF-MS was used to compare the contents of gingkolides and bibobalide in the leaves of Ginkgo biloba L. from different species in the southwest of Hubei province. The separation was performed on a ZORBAX C18 （2.1 ×20 ram,1.8 μm） column with acetonitrile and 0.1% formic acid in water as mobile phases at a flow-rate of 0.2 mL/min,and the column oven temperature 0f25 ℃. TOF mass spectrometry was used as the detector,and EIC mode was selected. The calibration curve was linear in the range of 0.2-4 μg/mL, with R2 being larger than 0.9996 and RSD being smaller than 1.65%. The developed method was rapid, selective, accurate and suitable for the quantitative analysis of gingkolides and bibobalide. Among the different species, the maximum contents of gingkolides and bibobalide were found in the Eng23 species. Ratio of gingkolides verus bibobalide in the leaves of EnG23 were better than others.