基于16SrDNA高通量测序方法检测猪舍空气微生物多样性

Microbial Diversity of Piggery Air Detected by 16S rDNA High-throughput Sequencing

采用16SrDNA高通量测序方法研究猪舍空气微生物的多样性。实验采用Illumina MiSeq 2×250系统,对细菌16S rDNA(V4)区进行测序;采用QIIME软件对数据进行分析。结果表明:猪舍空气微生物多样性丰富,基于97%的相似规律来确定OTU(operational taxonomical unit),其中,有16种门、115种科、217种属被鉴定;在属水平上,10种优势菌群是Pseudomonas、Acinetobacter、Streptococcus、Lactococcus、Enhydrobacter、Lactobacillus、Chryseobacterium、Wautersiella、Flavobacterium、Leuconostoc,其中,6种被报道为病原微生物。实验结果表明,了解猪舍空气微生物的多样性、丰度及优势菌群的病原性对猪舍疾病防控有一定指导意义。

Using 16S rDNA high-throughput sequencing to explore the microbial divers ity of the piggery air. Air samples were collected from 5 types of hoggeries in QiongLai. The V4 viriable region of 16s rDNA was amplified by PCR and sequenced with Illumina MiSeq 2×250 platform. QIIME software was used to analyze data. We observed high microbial diversity in piggery air. OTU （operational taxonomical unit） were identified based on the 97% similarity rule, which corresponds to, 16 bacteria Phylum, 115 Families and 217 Genus when balsted to known taxa from GreenGene data base. At Genus level, 10 abundant genus are Pseudomonas, Acinetobacter, Streptococcus, Lactococcus, Enhydrobacter, Lactobacillus, Chryseobacterium, Wautersiella, Flavobacterium, Leuconostoc, 6 of which among the top 10 were reported to be pathogenic. Study on the piggery air microbial diversity can provide a new guidance for animal disease prevention.