HPLC法快速测定人血浆中酒石酸美托洛尔的浓度

Rapid Determination of Metoprolol Tartrate Concentration in Human Plasma by HPLC

目的：建立快速测定人血浆中酒石酸美托洛尔浓度的方法。方法：血浆样品以3-叔丁基甲醚处理后,采用高效液相色谱法进样测定,色谱柱为Kromasil LC-18DB,流动相为乙腈-10 mmol/L辛烷磺酸钠溶液（p H=2.0）（30∶70）,柱温为25℃,流速为1.3ml/min,荧光检测波长为267 nm（激发波长）、290 nm（发射波长）,内标为吲哚洛尔。结果：酒石酸美托洛尔血药浓度在2~300 ng/ml范围内线性关系良好（r=0.999 9）,最低检测浓度为1 ng/ml。平均方法回收率为（101.13±4.0）%,日内RSD≤2.47%,日间RSD≤4.51%。结论：该方法简便、快速、灵敏、重现性好,适用于酒石酸美托洛尔临床血药浓度监测及人体药动学研究。

OBJECTIVE： To establish a method for rapid determination of metoprolol tartrate （MLT） concentration in human plasma. METHODS： Plasma sample was tested by HPLC after 3-tert-butyl methyl ether treatment. It was performed on a Kromasil LC-18DB column with the mobile phase consisted of acetonitrile -10 mmol/L octane sulfonate solution （pH=2.0） （30：70） at the flow rate of 1.3 ml/min with column temperature of 25℃ ; and the fluorescence detection wavelength was 267 nm （excitation wavelength）, 290 nm （emission wavelength）. RESULTS： The plasma concentration of MLT showed a good linear relationship in the range of 2-300 ng/mL （r=0.999 9） , and the minimum detectable concentration was 1 ng/ml. The mean recovery rate was （101.13 ±4.0）%, and RSDs of inra-day and interday were less than 2.47% and 4.51%, respectively. CONCLUSIONS： This method is found to be simple, rapid, sensitive, reproducible and applicable to the clinical plasma concentration monitoring of MLT and human pharmacokinetics study.