高效液相色谱-示差折光法测定饲料中低聚果糖含量

Determination of fructooligosaccharides in feeds by HPLC-RID

本实验建立了饲料中低聚果糖的高效液相色谱分离检测方法。试样用水超声萃取,以乙腈-水作为流动相,使用示差折光检测器进行检测。结果表明：蔗果三糖、蔗果四糖和蔗果五糖在0.1～5 mg/m L具有良好的线性关系,且相关系数均为1.0000;蔗果三糖、蔗果四糖和蔗果五糖检出限分别为0.03、0.04、0.05 g/kg,定量限分别为0.10、0.13、0.15 g/kg;方法的回收率为94.1%～108.1%,相对标准偏差为0.8%～5.1%。该方法定量准确可靠,重复性好,回收率高,适用于饲料中低聚果糖的日常检测。

A method for the determination of fructooligosaccharides in feeds by high performance liquid chromatography method of refractive index （HPLC-RID） was developed.The samples were vibrational extracted with water. The separation was carried out with a mobile phase of water-acetonitrile.The analytes were detected by refractive index detector.The results showed that there was a good linear correlation （R=1.0000）between the peak area and concentration of 1-kestose,Nystose and 1F-fruetofuranosylnystose within 0.1 - 5 mg/mL.The qualitative detection limit （LODs） of 1-kestose, Nystose and 1F- fructofuranosylnystose were 0.03,0.04,0.05 g/kg,and the quantitative detection limit （LOQs） were 0.10, 0.13 g/kg and 0.15 g/kg, respectively.The average recoveries for samples of fructooligosaccharides were 94.1% - 108.1%, and the relative standard deviations （RSDs） were in the range of 0.8% - 5.1%.The results obtained by experimental verification showed the method was simple,rapid and reliable for the determination of fructooligosaccharides in feeds.