甲醛对人绒毛滋养细胞毒性的研究

Toxic Effect of Formaldehyde on Human First Trimester Cytotrophoblasts

目的:研究甲醛对人绒毛膜滋养细胞活性及分泌功能的影响。方法:收集9例早孕人工流产患者的绒毛组织,原代培养绒毛膜滋养细胞,分别加入50,100,150,200μmol/L甲醛处理细胞48 h。用Cell Counting Kit-8(CCK-8)检测滋养细胞活性、放射免疫法检测人绒毛膜促性腺激素(h CG)水平。以DMEM/F12培养液为对照。结果:与对照组比较,100,150,200μmol/L甲醛染毒组的细胞活性显著抑制,差异有统计学意义(均P<0.05);各组之间h CG水平差异无统计学意义(P>0.05)。结论:甲醛达到一定浓度时(≥100μmol/L)对人绒毛膜滋养细胞的细胞活性有毒性作用。

Objective：To investigate the toxic effect of formaldehyde on the cellular viability and secretory function of human cytotrophoblasts at the first trimester stage. Methods：Human villi were obtained from nine patients undergoing abortion with the signed consent form. Cytotrophoblasts were extracted and cultured in vitro in medium, and then treated with formaldehyde （50,100,150,200 μmol/L） for 48 hours. The cellular viability of cytotrophoblasts was determined with Cell Counting Kit （CCK-8）, while the level of human chorionic gonadotropin （hCG） in medium was assayed with radioimmunoassay. DMEM/F12 medium was used as the negative control. Results：The cellular viabilities of cytotrophoblasts in three treatment groups with ≥100μmol/L of formaldehyde was significantly decreased than that in the control group （P0.05）. Conclusions：Formaldehyde at a higher dose （≥100μmol/L） may reduce the cellular viability of cytotrophoblasts.