低温射频等离子体降解乙腈中黄曲霉毒素B_1的效果与途径分析

Effect and Pathway Analysis of Aflatoxins B_1 in Degradation of Acetonitrile- Dissolved by Low Temperature Radio Frequency Plasma

采用低温射频等离子体处理溶解于乙腈中的黄曲霉毒素B1，考察了时间、等离子体发射功率、黄曲霉毒素B1初始浓度对黄曲霉毒素B1降解率的影响，通过HPLC—MS／MS分析降解产物及其可能的降解途径。结果表明，随着等离子体功率的增大、黄曲霉毒素B1初始浓度的降低，其降解率有增大的趋势。200～500μg／L黄曲霉毒素B1经120W等离子体处理150s以上，黄曲霉毒素B1的降解率可达95％以上。经过对比等离子体处理不同时间后的黄曲霉毒素B1的一级质谱，推测准分子离子峰157、299、339为可能的降解产物峰。结合产物峰的二级质谱碎片信息和黄曲霉毒素B1分子结构中的活性位点，推断出降解产物的结构和降解路径。

The effects of radiation duration, plasma transmit power, aflatoxins B1 initial concentration on the degradation rate of aflatoxins B1 dissolved in acetonitrile by low temperature radio frequency plasma were studied in this paper. Meanwhile, the possible degradation pathway and degradation products were analyzed by HPLC -MS/MS The results indicated that the aflatoxins B1 degradation rate was increased with elevated transmit power and reduced initial aflatoxins B1 concentration. When 200 ～ 500 μg/L aflatoxins B1 was exposed to 120 W plasma for more than 150 s, the degradation rate of aflatoxins B1 reached more than 95%. HPLC - MS/MS analysis of the degradation mixture revealed three major quasi - molecular peaks of 157,299, and 339 respectively after comparing first mass information of aflatoxins Bt through different plasma treatment time. The three peaks were proposed corresponding to the degradation products of aflatoxins B1. The degradation products and the pathway were identified using secondary mass fragments information and active site in the structure of aflatoxins B1.