山楂酸对腹泻型肠易激综合征的作用研究

Effect of Maslinic Acid on Diarrhea-predominant Irritable Bowel Syndrome

本文探究了山楂酸是否对腹泻型肠易激综合征有治疗作用,实验分空白组,模型组,阳性对照组和山楂酸高、低剂量共5组,运用乙酸-束缚联合刺激法建立了大鼠腹泻型肠易激综合征模型,检测分析了粪便含水量、腹腔回缩反射、离体肠肌动力、结肠组织病理染色、5-羟色胺免疫组化等多项体内体外指标。实验结果显示在体实验中,山楂酸高剂量组可明显改善大鼠稀便状况,且可使腹腔回缩反射恢复至正常范围,作用效果与阳性药相当,低剂量的山楂酸也表现出一定作用,但效果较弱;离体实验中,五组均无病理变化;高剂量山楂酸可恢复结肠对乙酰胆碱、5-羟色胺的正常敏感度,且使5-羟色胺免疫组化反应阳性的肠嗜铬细胞减少。本研究得出,山楂酸对腹泻型肠易激综合征有一定的治疗效果,且高剂量优于低剂量。

The efficacy of maslinic acid in the treatment of diarrhea-predominant irritable bowel syndrome （IBS-D） was investigated. Rats were divided into five groups： blank control, model, positive control, high-dose maslinic acid, and low-dose maslinic acid. The IBS-D model was set up by acetic acid-constraint-combined stimulation. Fecal water content, abdominal withdrawal reflex, in vitro colon contraction, and pathological staining and 5-hydroxytryptyptamine （5-HT） immunohistochemistry of colon were detected and analyzed. The in vivo results showed a significant improvement in the fecal water content; and the abdominal withdrawal reflex times returned to the normal range in the high-dose maslinic acid group. The effects were comparable to those of the positive control group. Low-dose maslinic acid exhibited weak activity. No pathological change was observed in any group in vitro. The susceptibility of colon to acetylcholine （Ach） and 5-HT was restored to normal in high-dose maslinic acid group, and 5-HT-positive enterochromaffin cells were reduced. These indicate that maslinic acid had certain efficacy for IBS-D, and high-dose maslinic acid displayed better effects than low-dose.