三种不同方法提取吴茱萸果实挥发油成分的GC-MS分析

Determination of the Constituents of Volatile oil from Evodiae Fructus Extracted with Three Different Exaction Methods by GC-MS

目的用GC-MS法分析吴茱萸果实中挥发油的化学成分,为其不同方法提取提供实验依据。方法采用水蒸气蒸馏法、索氏提取法和超声提取法对吴茱萸果实挥发油进行提取,用GC-MS法测定,结合NIST05数据库对分离的化合物进行鉴定,并采用归一法计算各组分的相对峰面积。结果 3种不同方法提取吴茱萸果实挥发油的化学成分有差异,分别分离鉴定出57、51和38个组分,共有成分25个,其中主要成分为β-月桂烯、β-罗勒烯、α-蒎烯、β-芳樟醇、[1S-(1α,2β,4β)]-1-乙烯基-1-甲基-2,4-二(1-甲基乙烯基)-环己烷、石竹烯、吉马烯、α-布藜烯和氧化石竹烯等。结论采用水蒸气蒸馏法和索氏提取法得到具有一定药理活性的石竹烯、α-蒎烯、榄香烯及β-罗勒烯等成分时,能够达到同样的提取效果。因此可以综合考虑所需要的组分而选择合适的提取方法。

Objective To analyze the chemical components of the essential oil of from Evodiae Fructus with GC-MS and provide experimental basis for its different exaction methods. Methods The volatile oils of Evodiae Fructus were extracted by steam distillation, Soxhlet extraction and ultrasonic extraction methods. The chemical compounds were identified by GC- MS method and NIST05 database. The relative content of each component was calculated by area normalization. Results There are some differences in chemical compounds of the essential oil from Evodiae Fructus by three different methods. 57,51 and 38 components of volatile oil were identified separately, and there were 25 same components. The main components are β- Myrcene, β-ocimene, α-pinene, β-Linalool, [1S-（lα,213,4[3）]-l-ethenyl-l-methyl-2,4-bis （1-methylethenyl）-cyclohexane, caryophyllene, germacrene D, α-bulnesene and caryophyllene oxide etc. Conclusion The steam distillation and Soxhlet extraction methods showed the same effect in extracting of chemical compounds with some pharmacological activities, such as caryophyllene,α-pinene,elemene and β-oeimene etc. So the needs of components should be taken into consideration for the right extraction method.