转铁蛋白水平与男性生育及睾丸足细胞功能的关系

Relationship between transferrin levels and function of male fertility and testicular podocyte

目的探讨转铁蛋白(Tf)水平与男性生育及睾丸足细胞功能的关系。方法收集临床男性不育症患者和正常生育者的精液标本,采用精子质量分析仪进行精子密度及活动率分析,并检测精液Tf水平;无菌切取大鼠睾丸,经胶原酶及透明质酸酶消化,分离出纯度较高的足细胞并培养,测定细胞培养液Tf水平;Tf水平测定均采用免疫速率散射比浊法。结果男性不育症患者精液Tf水平[(15±5)μmol/L]低于正常生育者[(24.5±6.5)μmol/L,P<0.01],而且与精子密度和活动率呈正相关(P<0.01)。正常生育组大鼠睾丸足细胞悬液Tf水平[(25±8)μmol/L]高于不育组[(15±6)μmol/L,P<0.01]。结论精液Tf水平的测定可作为反映足细胞功能,评价曲细精管生精功能及精子质量的指标,对男性不育症的诊断、治疗具有重要的价值。

Objective To investigate the relationship between levels of transferring（Tf）and function of male fertility and testicular podocyte.Methods Semen samples from male patients with infertility and volunteers with normal infertility were collected and tested for sperm density and motility by using sperm quality analyzer.In additon to that,Tf concentrations of Tf were determined;aseptically excised rat testis and by collagenase and hyaluronidase digestion,podocytes were isolated with high purity and cultured in culture medium,then the concentration of Tf in cell culture medium was determined;Tf concentration were determined by rate nephelometry.Results Tf concentration in semen of male-infertility patients[（15±5）μmol/L]was significantly lower than that of normal-fertility volunteers[（24.5±6.5）μmol/L,P〈0.01],which were positively correlated sperm count and motility（P〈0.01）.Tf concentrations of cell culture medium[（25±8）μmol/L]in which testicular podocytes were cultured were significantly higher than that of infertile rat[（15±6）μmol/L,P〈0.01].Conclusion Determination of semen Tf levels can reflect podocyte function and be used as an indicator for the evaluation of seminiferous tubules spermatogenesis,which was valuable in the diagnosis and treatment of male infertility.