抗Dia合并抗E抗体致交叉配血不合的临床分析

Case analysis of incompatibility in blood crossmatching induced by anti-Dia and anti-E antibodies

目的探讨交叉配血中抗Dia合并抗E抗体致配血不合的原因。方法对该院1例因抗Dia合并抗E抗体致多次配血不合的患者进行分析。通过血型鉴定、直接抗人球蛋白试验、不规则抗体筛查、吸收放散实验来确定患者的血型、可能的不规则抗体、细胞谱反应格局。结果患者血型正定型:抗A抗体(-)、抗B抗体(-);反定型:Ac(++++)、Bc(++++)、RBC(-)、Oc(-);Rh血型定型:抗c抗体(-)、抗C抗体(++++)、抗D抗体(++++)、抗e抗体(++++)、抗E抗体(-)。直接抗人球蛋白试验(-)。患者血清中含有抗E、抗c抗体。结论患者血清中的不规则抗体是导致交叉配血不合的重要原因。

Objective To investigate the reason of anti-Dia combined with anti-E antibodies inducing incompatibility in blood crossmatching.Methods Retrospectively analyse a case of patient treated in the hospital,whose blood crossmatching failed for several times.Through blood typing,direct Coombs test,irregular antibody screening,adsorption and elution experiments to determine the patient′s blood type,possible irregular antibodies,cell spectral response pattern.Results Forward blood typing：anti-A antibody（-）,anti-B antibodies（-）;Reverse blood typing：Ac（＋＋＋＋）,Bc（＋＋＋＋）,RBC（-）,Oc（-）;Rh blood grouping：anti-c antibody（-）,anti-C antibody（＋ ＋ ＋ ＋）,anti-D antibody（＋ ＋ ＋ ＋）,anti-e antibody（＋ ＋ ＋ ＋）,anti-E antibody（-）,direct Coombs test（-）.Patient′s serum contained anti-E and anti-c antibodies.Conclusion Irregular antibodies in the serum of patients is an important cause of crossmatch discord.