葡萄皮渣中原花青素对临床常见分离菌的抗菌效应分析

Antibacterial effect of grape peel procyani residudins on common clinical bacteria

目的探讨葡萄皮渣中的原花青素对临床常见分离菌大肠杆菌、铜绿假单胞菌、鲍曼不动杆菌、肠球菌及耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌作用。方法应用琼脂平板法测定原花青素对临床分离出的30株大肠杆菌、28株铜绿假单胞菌、15株鲍曼不动杆菌、32株屎肠球菌、25株粪肠球菌、70株MRSA的最低抑菌浓度（MIC）,并计算半数抑菌浓度（MIC50）和90%抑菌浓度（MIC90）,用统计软件SPSS16.0对结果进行统计分析。结果 3-8mg/mL的原花青素对大肠杆菌、铜绿假单胞菌、鲍曼不动杆菌等革兰阴性杆菌均无抑菌作用;3-8mg/mL的原花青素对粪肠球菌无抑菌作用,对屎肠球菌的抑菌率仅为6.3%;用原花青素对70株MRSA进行抑菌检测：4mg/mL原花青素的抑菌率为65.7%,8mg/mL抑菌率为95.7%,MIC50为4.221mg/mL、MIC90为6.260mg/mL。结论葡萄皮渣中的原花青素对常见革兰阴性杆菌如大肠杆菌、铜绿假单胞菌及鲍曼不动杆菌无抑菌作用,对革兰阳性球菌如MRSA、肠球菌有一定的抑菌效果,特别是对MRSA抑菌作用非常明显（P〈0.05）。

Objective To investigate the antibacterial effect of grape peel residue procyanidins on common clinical bacteria,including E.coli,Pseudomonas aeruginosa,Acinetobacter baumannii,Enterococcus and methicillin-resistant Staphylococcus aureus（MRSA）.Methods By the means of agar plate method,we detected the minimum inhibitory concentration（MIC）of 30 strains of E.coli,28 strains of Pseudomonas aeruginosa,15 strains of Acinetobacter baumannii,32 strains of Feces Enterococcus,25 strains of E.faecalis and 70 strains of MRSA,then calculate the MIC50 and MIC90.The result was analyzed with SPSS software 16.0.ResultsThe concentration of procyanidins at 3-8mg/mL had no inhibitory effect on E.coli,Pseudomonas aeruginosa and Acinetobacter baumannii;there was no inhibitory effect on E.faecalis at 3-8mg/mL,but the inhibition rate to Feces Enterococcus was 6.3%.By measuring the inhibitory effect of procyanidins on 70 MRSA,the inhibition rate at 4mg/mL was 65.7%,at 8mg/mL was 95.7%,MIC50 was 4.221mg/mL and MIC90 was 6.260mg/mL.Conclusion There are no inhibitory effects of grape peel residue procyanidins on Gram-negative bacilli such as E.coli,Pseudomonas aeruginosa,Acinetobacter baumannii,and there are certain inhibitory effects on Gram-positive bacteria such as MRSA,enterococci,especially on MRSA（P〈0.05）.