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人乳腺癌细胞微球体生物学特性研究 被引量：3

Study on the biological characteristics of human breast cancer cell mammospheres
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摘要 目的研究人乳腺癌细胞微球体(MSs)的生物学特性,建立乳腺癌干细胞实验模型。方法无血清悬浮培养人乳腺癌MCF-7细胞(MCF-7组),并获取MSs(MSs组)。利用细胞划痕实验、transwell实验及动物成瘤实验检测MSs在细胞迁移、侵袭性生长及体外成瘤等方面的生物学特性。结果细胞划痕实验提示:MSs组划痕带可在48h后愈合,而MCF-7组划痕带在48h后未能愈合;transwell实验提示MSs组中可见到(76.24±0.35)个细胞通过生物膜,而MCF-7细胞组中为(17.38±0.18)个(P<0.05);动物成瘤实验提示:MSs在成瘤速度及移植瘤体积方面均强于MCF-7细胞。结论 MSs具有极强的迁移、侵袭性生长及动物体内成瘤的能力,可作为实验模型应用于乳腺癌干细胞相关研究中。 Objective To study the biological characteristics of the human breast cancer cell mammospheres（MSs）,and construct breast cancer stem cell experiment model.Methods MCF-7cells were cultured in the serum-free media supplemented with growth factors（the MCF-7group）,and the MSs was collected（the MSs group）.The migration,invasive and animal tumor formation abilities of MSs were detected by wound healing,transwell invasive assay and animal tumor formation test.Results The wound line of MSs healed after 48 hours,but the line of MCF-7cells could not heal after 48 h.The number of the cells going through the membrane in MSs group was（76.24±0.35）,and the number in MCF-7cells was（17.38±0.18）（P〈0.05）.MSs had stronger animal tumor formation ability than MCF-7cells.Conclusion MSs have stronger abilities in migration,invasive and animal tumor formation,and could be used in the studies of breast cancer stem cell as experimental model.
作者 范原铭 侯婧 董宁 王强 顾敏
机构地区 重庆市长寿区人民医院普外科 重庆市长寿区人民医院肿瘤科 重庆市长寿区妇幼保健院外科
出处 《重庆医学》 CAS 北大核心 2015年第4期456-458,461,共4页 Chongqing medicine
关键词 乳腺肿瘤 肿瘤干细胞 无血清培养 breast neoplasms neoplastic stem cell culture in serum-free media
分类号 R737.9 [医药卫生—肿瘤]
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参考文献15

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共引文献5

同被引文献36

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二级引证文献4

重庆医学
2015年 第4期

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