过敏性哮喘患者外周血细胞因子水平变化及临床意义

The clinical change of cytokines expression in peripheral blood in patients with allergic asthma

目的探讨细胞因子IL-17、IL-22和IL-10在过敏性哮喘患者外周血中的变化及临床意义。方法选取过敏性哮喘患者40例(哮喘组)和健康体检者30例(对照组),采用ELISA法检测外周血培养上清中IL-17、IL-22和IL-10的表达;电化学发光免疫分析法检测血清中IgE的水平,全自动血液分析仪检测全血嗜酸性粒细胞比率;IL-17、IL-22和IL-10与哮喘严重程度进行Pearson相关性分析。结果哮喘组血清中IgE的水平及全血嗜酸性粒细胞比率高于对照组(P<0.05);哮喘组外周血IL-17、IL-22水平高于对照组,而IL-10水平低于对照组(P<0.05);哮喘严重程度与IL-17、IL-22水平呈正相关(P<0.01),而与IL-10水平呈负相关(P<0.05)。结论 IL-17、IL-22和IL-10在过敏性哮喘的发病机制中起重要作用,动态监测其变化有利于临床诊断及治疗。

Objective To observe the clinical change of cytokines expression in peripheral blood in patients with allergic asthma and its significance.Methods Forty patients with allergic asthma and thirty healthy controls were enrolled.The concentrations of IL-17,IL-22 and IL-10 in peripheral blood were measured by ELISA;the serum IgE levels were measured by electrochemical luminescence method;The eosinophil proportion was detected by blood cell analyzer;the IL-17,IL-22,IL-10 and the severity of asthma were conducted Pearson correlation analysis.Results The level of serum IgE and eosinophil proportion was significantly higher in patients with allergic asthma compared with the controls（P〈0.05）;compared with the control group,the concentration of IL-17 and IL-22 in peripheral blood of patients with allergic asthma were increased,but the expression of IL-10 were decreased（P〈0.05）;the level of IL-17 and IL-22 were positively correlated with severity of allergic asthma（P〈0.01）,but the level of IL-10 was negatively correlated with severity of allergic asthma（P〈0.05）.Conclusion The expression of IL-17,IL-22 and IL-10 play an important role in the pathogenetic mechanism of allergic asthma,and a dynamic monitoring could be helpful for the diagnoses and treatment of allergic asthma.