鹿茸提取物复方对骨质疏松症调节作用

Effect of pilose antler compound extraction on glucocorticoid-induced osteoporosis in rats

目的探讨鹿茸提取物复方对糖皮质激素性骨质疏松症(GIO)大鼠脂类代谢指标影响及机制。方法将79只Wistar大鼠随机分为对照组、模型组、鹿茸组及骨疏康组,采用后肢肌注地塞米松(2.5 mg/kg,每周2次)造模,造模同时,鹿茸组(0.439 g/kg)及骨疏康组(2.1 g/kg)灌胃处理,连续9周,采用骨密度仪测定大鼠股骨骨密度、自动生化分析仪测定血清高密度脂蛋白胆固醇(HDL-C)含量、实时定量PCR法及Western blot法测定骨组织脂肪细胞型脂肪酸结合蛋白(A-FABP)mRNA和蛋白表达。结果对照组、模型组、鹿茸组及骨疏康组大鼠股骨骨密度分别为(0.116±0.009)、(0.108±0.006)、(0.115±0.009)、(0.111±0.006)g/cm2,血清HDL-C含量分别为(0.503±0.013)、(0.352±0.017)、(0.496±0.023)、(0.383±0.015)mmol/L;与对照组比较,模型组大鼠股骨骨密度、血清HDL-C含量明显降低(P<0.01),股骨A-FABP mRNA和蛋白表达明显增强(P<0.01);与模型组比较,鹿茸组大鼠股骨骨密度、血清HDL-C含量明显升高(P<0.01),股骨A-FABP mRNA及蛋白表达明显降低(P<0.01)。结论鹿茸提取物复方对大鼠GIO具有一定干预作用,其机制可能与降低大鼠血清中HDL-C含量、升高骨组织A-FABP表达有关。

Objective To explore the effect of pilose antler compound extraction on glucocorticoid-induced osteoporosis （GIO）. Methods Seventy-nine Wistar rats were divided randomly into four groups：normal group,model group,pi- lose antler compound extraction group, and Gushukang group. Rat GIO model was established by intramuscular injection of dexamethasone at the dose of 2. 5 mg/kg, two times a week for 9 weeks and the rats in pilose antler compound extrac- tion group and Gushukang group were administered with pilose antler compound extraction at the dose of 0. 439 g/kg and Gushukang granule at the dose of 2. 1 g/kg, respectively, at the same time by gavage, and 10 ml/kg normal saline was administered to the rats of model and control group. After the 9 weeks＇ treatment, the bone density of femur of all the rats was measured with dual energy X-ray bone density instrument;the concentration of serum high density lipoprotein choles- terol（HDL-C） was detected with automatic biochemistry analyzer and the expressions of adipocyte fatty acid binding pro- tein（A-FABP） and A-FABP mRNA in the femur with Western blot and real-time PCR method. Results The average bone mineral density （BMD） of the rats in normal group, model group, pilose antler compound extraction group, and Gushukang group were 0. 116 ＋0. 009,0. 108 ±0. 006,0. 115 ±0. 009,and 0. 111 ±0. 006 g/cm2 ,and the average serum HDL-C were 0. 503 ± 0. 013,0. 352 ± 0. 017,0. 496 ± 0. 023, and 0. 383 ＋ 0. 015 mmo/L, respectively. Compared with those of the normal group,BMD and HDL-C content in model group were markedly reduced（P 〈0.01 ） ,while the ex- pressions of A-FABP and A-FABP mRNA in model group were increased markedly（ P 〈 0. 01 ）. Compared with those of the model group, BMD and HDL-C content in pilose antler compound extraction group were markedly increased （P 〈 0. 01 ）, while the expressions of A-FABP and A-FABP mRNA were markedly reduced（ P 〈 0. 01 ）, and the indica- tors of pilose antler compound extraction group were better than those of the Gushukang group. Conclusion Pilose antler compound extraction has intervention effect on GIO in rats and the effect may be related to the decrease of serum HDL-C and the increase of A-FABP expression in bone tissue.