蓝莓花色苷对环磷酰胺致大鼠心脏损伤保护作用

Protective effect of blueberry anthocyanins on cyclophosphamide-induced cardiac injury in rats

目的观察蓝莓花色苷对环磷酰胺致大鼠心脏毒性的保护作用,并探讨其作用机制。方法雄性SD大鼠32只,随机分为对照组、环磷酰胺组和蓝莓花色苷20、80 mg/kg组,连续灌胃14 d后,处死动物,其中第8天环磷酰胺组及蓝莓花色苷组大鼠腹腔注射环磷酰胺100 mg/kg;观察心肌组织病理学改变,采用自动生化分析仪测定大鼠血清酶学指标,采用试剂盒法检测心肌组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,Western blot检测心肌细胞Bcl-2和Bax蛋白表达水平。结果环磷酰胺组大鼠心脏出现心肌细胞肥大、变性坏死、凋亡及炎性细胞浸润等病理变化,而蓝莓花色苷组大鼠心脏病理变化不同程度减轻;与对照组比较,环磷酰胺组大鼠心肌细胞乳酸脱氢酶(LDH)、天门冬氨酸氨基转换酶(AST)、肌酸激酶活性[分别为(314.7±9.2)、(772.5±18.3)、(501.5±13.6)U/L]明显升高,心肌组织SOD活性[(21.2±1.4)U/mg prot]下降,MDA含量[(171.2±12.4)μg/gprot]升高;与环磷酰胺组比较,蓝莓花色苷80 mg/kg组大鼠心肌细胞LDH、AST、肌酸激酶活性[分别为(127.7±7.4)、(411.5±10.3)、(268.6±8.4)U/L]明显下降,心肌组织SOD活性[(26.9±1.3)U/mg prot]升高,MDA含量[(144.9±12.8)μg/g prot]下降。结论蓝莓花色苷对环磷酰胺致大鼠心脏毒性具有一定保护作用,作用机制可能与抗氧化有关。

Objective To observe protective effect of blueberry anthocyanins on cyclophosphamide-induced cardio- toxicity in rats and to explore the mechanism of the effect. Methods Thirty-two male Sprague Dawley rats were ran- domly divided into control group, model group and blueberry anthocyanins group （20 mg/kg and 80 mg/kg）. All animals in treatment groups were administerted with blueberry anthocyanins by gavage once a day for 14 days continuously. The rats in the model group and treatment group were injected with cyclophosphamide（ 100 mg/kg）at 8th day of the experi- ment. Myocardial histopathology change was observed. Malondialdehyde （MDA） content and superoxide dismutase （SOD） activity in serum of the rats were detected by using kit assay automaticbiochemical analyzer. Bcl-2 and Bax pro- tein levels of myocardial cells were detected with Western blot method. Results The rats in the model group showed cardiac myocyte hypertrophy, degeneration/necrosis, apoptosis, and infiltration. Whereas the extent of cardial myocyte de- generation and necrosis, apoptosis, and infiltration were alleviated in blueberry anthocyanins group. Myocardial cell injury indices such as lactate dehydrogenase （314. 7 ± 9.2 U/L）, aspartate aminotransferase （772. 5 ± 18.3 U/L）, and creatine kinase （501.5 ± 13.6 U/L） increased significantly in model groups, while the indexes decreased significantly in blueberry anthocyanins treatment group（ 127.7 ± 7.4,411.5 ± 10. 3, and 268.6 ± 8.4 ）. The content of SOD decresead and MDA content increased significantly in myocardial tissue of model group, wheras in blueberry anthocyanins group, SOD level increased and MDA content decreased significantly. Conclusion Blueberry anthocyanins have protective effect on cyclo- phosphamide-induced cardiac toxicity in rats and the mechanism of the effect may be related to antioxidant capacity of blueberry anthocyanins.