富锗灵芝对四氯化碳诱导大鼠肝损伤保护作用

Protective effect of Ge-enriched Ganoderma lucidum on CCl4-induced hepatic injury in rat

目的探讨富锗灵芝胶囊对四氯化碳(CCI4)所致大鼠肝损伤的保护作用。方法 50只Wistar雄性大鼠,随机分为对照组、模型组、富锗灵芝高、中、低剂量组(分别灌胃给予富锗灵芝胶囊450、230、80 mg/kg),采用CCl4诱导大鼠急性肝损伤;分别测定血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,肝组织经苏木素-伊红染色,光镜下观察肝组织病理状况,并计算肝损伤指数。结果与对照组比较,模型组大鼠ALT、AST活性[分别为(258.00±64.81)、(423.60±139.85)IU/L]明显升高,肝脏病变总评分[(194.50±27.71)分]明显升高(P<0.01);与模型组比较,富锗灵芝中、低剂量组大鼠血清ALT、AST活性[分别为(131.30±98.94)、(264.00±187.02)和(138.50±86.08)、(265.70±140.59)IU/L]明显降低(P<0.01),大鼠肝脏病变总评分[分别为(141.40±48.35)和(134.10±25.89)分]明显降低(P<0.01)。结论富锗灵芝胶囊对CCl4所致大鼠肝损伤具有一定保护作用。

Objective To observe the protective effect of germanium （Ge）-enriched Gnoderma lucidum on tetra- chloromethane（CCl4）-induced acute hepatic injury in rats. Methods Fifty rats were divided into 5 groups （normal group ,CCl4 model group ,high-,moderate-, and low-dose groups with Ge-enriched Gnoderma lucidum at the dosages of 450,230, and 80 mg/kg via gavage）. The rat acute hepatic injury model was induced by CCl4. The serum level of alanine transaminase（ALT） and aspartate transaminase（AST） were determined and pathologic changes of hepatic tissues were ex- amined. Results Compared with those of the rats in the control gropu, the serum ALT and AST and the liver damage in the rats of the experimental hepatic injury groups were markedly decreased after the administration of moderate and low dose of Ge-enriched Ganoderma lucidum. Conclusion Ge-enriched Ganoderma lucidum has protective effect on CCl4- induced hepatic injury in rat.