脾氨肽对支气管哮喘相关细胞因子调控的研究

Research on bronchial asthma-related cytokine regulation by spleen aminopeptidase

目的研究脾氨肽对IL-10、IL-5和肿瘤坏死因子α（TNF-α）的调控作用及机制。方法分离人外周血CD4＋T细胞和树突状细胞（DC）体外共培养，用鸡卵白蛋白（0VA）作为抗原激活T细胞，48h后收集上清用酶联免疫吸附试剂盒检测细胞因子的变化，并且使用P13K抑制剂探讨该通路对IL-10的调节作用。结果不加0VA组检测到微量的IL-10、IL-5以及TNF—Ct的分泌，加入OVA组CD4＋T细胞和DC体外共培养可以检测到IL-10、IL-5以及TNF-α的分泌，脾氨肽可以促进IL-10的分泌，但对IL-5和TNF-α起抑制作用。P13K信号通路在IL-10的分泌中起了重要作用。结论脾氨肽在体外可以促进IL-10，抑制IL-5和TNFa的分泌，P13K通路在IL-10的分泌中起了重要作用。

Objective To study the spleen aminopeptidase regulatory role for IL-10, IL-5 and TNF-α production and modulatory mechanism for IL 10. Methods CD4＋ T cells and dendritic cells （DC） were isolated from human peripheral blood and co-cultured with chicken ovalbumin （OVA） as antigen to activate T cells, supernatants were collected after 48 hours, cytokines were detected by enzyme-linked immunoassay kit,and also investigated PI3K pathway regulating function on IL-10 using PI3K inhibitors. Results The trace amounts of IL-10,IL-S and TNF-α secretion were detected in without OVA group, IL-10,IL-5,and the secretion of TNF-α were detected after CD4＋ T cells and DC co-culture in joining OVA group, and the spleen aminopeptidase can promote the secretion of IL-10, but play an inhibitory effect on IL-5 and TNF-α secretion. PI3K signaling pathway plays an important role in the secretion of IL-10. Conclusions Spleen aminopeptidase can promote in IL-10 secretion in vitro,inhibit the secretion of IL-5 and TNF-α,PI3K pathway plays an important role in the secretion of IL-10.