CCR2和CCR5基因多态性与结核性脓胸的关系

Association of CCR2 gene and CCR5 gene polymorphisms with the risk of tuberculous empyema

目的探讨CCR2基因-190 G/A和CCR5基因-59029 G/A单核苷酸多态性(SNP)与结核性脓胸的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对结核性脓胸患者300例和健康对照组300例进行CCR2基因-190 G/A和CCR5基因-59029 G/A SNPs检测。结果与CCR2基因-190 GG基因型相比,携带AG和AA基因型均可增加结核性脓胸发病风险(OR=2.254,95%CI 1.043~4.868;OR=8.728,95%CI 4.224~18.033)。进行分层分析发现,无卡介苗(BCG)接种史、体质量指数(BMI)<18.5 kg/m^2均增加结核性脓胸发病风险(OR=3.309,95%CI 2.108~4.382;OR=2.767,95%CI 1.918~3.993)。CCR5基因-59029 G/A各基因型未入选回归方程,无统计学意义(P>0.05)。结论CCR2基因-190 G/A SNP可能与结核性脓胸的发病风险有关;CCR5基因-59029 G/A SNP可能与结核性脓胸无关。

Objective To investigate the relationship of CCR2 gene-190 G/A, CCR5 gene-59029 G/A single nucle-otide polymorphism （ SNP） and tuberculous empyema. Methods Using polymerase chain reaction restriction fragment length polymorphism （ PCR-RFLP） method, 300 cases of tuberculous empyema patients and 300 healthy control were received 190 G/A and 59029 G/A SNPs detection. Results Compared to CCR2 gene-190 GG genotype, carrying AG and AA genotype can increase the risk of tuberculous empyema （ OR =2. 254, 95%CI 1. 043 -4. 868; OR =8. 728, 95%CI 4. 224 -18. 033）. Stratified analysis revealed that, without Bacillus Calmette Guerin （BCG） vaccination history, body mass index （BMI） 〈18. 5 kg/m^2 were all increased the risk of tuberculous empyema （OR=3. 309, 95%CI 2. 108 -4. 382; OR=2. 767, 95%CI 1. 918-3. 993）. CCR5 gene-59029 G/A genotypes were not selected in the regression equation, no statisti-cal significance was found （ P 〉0. 05）. Conclusion CCR2 gene-190 G/A SNP might be related to the tuberculous empye-ma. CCR5 gene-59029 G/A SNP may have no association with tuberculous empyema.