摘要
目的探讨缺血后处理(IPO)对肝硬化大鼠肝脏缺血-再灌注损伤(IRI)的保护作用及其免疫机制。方法按随机数字表法将30只肝硬化模型SD大鼠随机分为IPO组、IRI组和单纯肝切除组(肝切组),各10只。IPO组先切除40%肝脏,阻断第一肝门20 min,然后反复缺血-再灌注3次,最后持续再灌注;IRI组,切除40%肝脏,阻断第一肝门20 min后持续再灌注;肝切组切除40%肝脏。分别于术后6、24 h抽取大鼠下腔静脉血,检测ALT、AST、分化群(CD)4+、CD8+、调节性T细胞(Treg)百分率、白细胞介素(IL)-4、IL-10水平。3组比较采用单因素方差分析,两两比较采用LSD-t检验。结果恢复灌注6 h后,IPO组的ALT、AST分别为(1 623±378)、(1 993±469)U/L,IRI组相应为(2 690±549)、(3 020±577)U/L,IPO组ALT、AST明显低于IRI组(LSD-t=-4.21,-3.72;P<0.05)。恢复灌注24 h后,IPO组的ALT、AST分别为(307±76)、(555±137)U/L,IRI组相应为(518±105)、(1 050±355)U/L,IPO组ALT、AST亦明显低于IRI组(LSD-t=-4.06,-3.37;P<0.05)。恢复灌注6 h后,IPO组CD4+、CD8+、CD4+/CD8+比值、Treg、IL-4、IL-10分别为(57±5)%、(25±3)%、2.3±0.5、(8.9±0.4)%、(1.27±0.25)mg/L、(0.61±0.03)mg/L,IRI组相应为(52±6)%、(12±3)%、4.5±0.8、(7.3±0.3)%、(0.66±0.11)mg/L、(0.34±0.06)mg/L,IPO组CD8+、Treg、IL-4、IL-10较IRI组明显升高,CD4+/CD8+比值明显降低(LSD-t=7.74,6.67,5.52,9.31,-6.69;P<0.05)。结论 IPO可能通过减轻免疫损伤发挥对肝硬化大鼠肝脏IRI的保护作用。
ObjectiveTo investigate the protective effects and its mechanism of ischemia postconditioning (IPO) in hepatic ischemia-reperfusion injury (IRI) in liver cirrhotic rats.MethodsThirty liver cirrhotic Sprague-Dawley (SD) rats were randomly divided into three groups according to the random table methods: ischemia postconditioning (IPO) group, IRI group, and pure hepatectomy (PH) group with 10 rats in each group. Rats in IPO group underwent partial hepatectomy (40%), and the first portal was occluded for 20 min, then 3 times of ischemia-reperfusion were performed, and ifnally continuous&nbsp;reperfusion. Rats in IRI group underwent partial hepatectomy (40%), and the ifrst portal was occluded for 20 min, then continuous reperfusion was performed. Rats in PH group underwent partial hepatectomy (40%) only. Inferior vena cava blood was collected at 6, 24 h after operation. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), proportion of cluster of differentiation (CD) 4+, CD8+, regulatory T cells (Treg) and levels of interleukin (IL)-4, IL-10 were tested. Comparison of three groups was conducted by one way analysis of variance and pairwise comparison by LSD-t test.ResultsAfter 6 h reperfusion, levels of ALT and AST in IPO group [(1 623±378) , (1 993±469) U/L] were signiifcantly lower than those in IRI group [(2 690±549) , (3 020±577) U/L] (LSD-t=-4.21,-3.72; P〈0.05). After 24 h reperfusion, levels of ALT and AST in IPO group [(307±76) , (555±137) U/L] were still signiifcantly lower than those in IRI group [(518±105) , (1 050±355) U/L] (LSD-t=-4.06,-3.37;P〈0.05). After 6 h reperfusion, proportion of CD4+, CD8+, CD4+/CD8+ ratio, Treg, and levels of IL-4, IL-10 in IPO group were (57±5) %, (25±3) %, 2.3±0.5, (8.9±0.4) %, (1.27±0.25) mg/L, (0.61±0.03) mg/L, respectively. While in IRI group, they were (52±6)%, (12±3) %, 4.5±0.8, (7.3±0.3) %, (0.66±0.11) mg/L, (0.34±0.06) mg/L, respectively. In IPO group, CD8+, Treg, IL-4 and IL-10 increased signiifcantly, while CD4+/CD8+ ratio decreased signiifcantly, compared with those in IRI group (LSD-t= 7.74, 6.67, 5.52, 9.31,-6.69;P〈0.05).ConclusionIPO can prevent from hepatic IRI injury in liver cirrhotic rats through decreasing the immune injury.
出处
《中华肝脏外科手术学电子杂志》
CAS
2014年第6期36-39,共4页
Chinese Journal of Hepatic Surgery(Electronic Edition)
基金
国家自然科学基金(81201918)
广东省自然科学基金(10151008901000113)