3种生长延缓剂对彩色马蹄莲无菌苗生长的影响

Effects of 3 plant growth retardants on growth of in vitro Zantedeschia aethiopica cv.hybrida

使用不同浓度多效唑（PP333）、矮壮素（CCC）、水杨酸（SA）结合不同浓度配比的6 BA和NAA以促进彩色马蹄莲无菌侧芽分化和生长。将用不同浓度的PP333、CCC、SA处理后的彩色马蹄莲无菌苗转入MS＋6‐BA 2．0 mg／L培养基中进行增殖培养，结果表明：PP333对抑制植株生长、促进块茎膨大及芽分化具有明显效果，并以8．0 mg／L为最佳浓度。用含不同浓度NAA、6 BA的MS培养基培养经PP3338．0 mg／L处理过的彩色马蹄莲无菌苗，最佳增殖培养基为MS＋6 BA2．0～3．0mg／L。

With different combinations of 6 BA and NAA ,the effects of PP333 ,CCC and SA at different concentrations on the growth differentiation of in vitro Z .aethiopicacv .hybrida were studied .After being cultured by PP333 ,CCC and SA at different concentrations ,the in vitro Zantedeschia aethiopica cv .hybrida .was transferred into the MS medium supplemen‐ted with 6 BA 2 .0 mg/L for multiplication .The result showed that paclobutrazol ,especial‐ly with the optimum concentration of 8 .0 mg/L ,had a significant effect on increasing the mi‐cro‐tuber size and promoting the shoot multiplication as well as on inhibiting the plantlet height and leaf number .In vitro Z .aethiopica cv .hybrida .treated with PP333 8 .0 mg/L , was cultured on the MS medium supplemented with NAA and 6 BA at different concentra‐tions ,with the optimum concentration of 6 BA from 2 .0 mg/L to 3 .0 mg/L .