不同连翘制剂对甲型流感病毒NP基因转染后表达的影响

Effect of Different Forsythia Preparations on Influenza Virus Nucleoprotein Gene Expression after Transfection

目的:观察连翘苷、连翘醇提液、连翘水煎剂等3种连翘制剂对甲型流感病毒甲1型流感病毒核蛋白(nucleoprotein,NP)基因转染后表达的影响。方法:将甲型流感病毒NP基因转染Hela细胞,用甲型流感病毒胶体金检测3种连翘制剂对转染后细胞内和上清核蛋白表达情况,用RT-PCR检测转染后Hela细胞内NP基因的拷贝数。结果:NP重组质粒组上清胶体金检测为阳性,连翘苷组上清为阴性、细胞内为弱阳性;连翘水煎剂及连翘醇提物组上清均为弱阳性。连翘苷组NP基因表达量较NP重组质粒组减少(P<0.05)。结论:连翘苷抑制甲型流感病毒NP基因转染后表达作用最强。

Objective：To investigate the effect of phillyrin, forsythia ethanol-extraction liquid and forsythia water decoction on influenza virus nucleoprotein（NP） gene expression. Methods：Transfect the Hela cells with influenza virus NP gene. Influenza virus colloidal gold was used to detect the three kinds of forsythia preparations supernatant and intracellular NP expression. RT-PCR was used to detect the NP gene copy number. Results ： The colloidal gold test results of the NP gene recombination plasmid group were positive, phillyrin group supernatant were negative, intracellular were weak positive. Forsythia water decoction and forsythia ethanol-extraction liquid supernatant were weak positive. The NP gene copy number of phillyrin group was decreased compared with group of NP gene recombination Plasmid （ P 〈 0.05 ）. Conclusion ： Phillyrin had the strongest effect of the three preparations to inhibit influenza viruses NP gene expression after transfection.