嗜酸氧化亚铁硫杆菌培养及其影响因素

The Cultivation of Acidithiobacillus Ferrooxidans and the Investigation of Influencing Factors

研究了温度、pH值、溶氧量(以转速表征)、金属离子浓度等因素对氧化亚铁硫杆菌的生长状况及体内ATP酶活力的影响,确定了最适的培养条件.通过对比培养前与培养后微生物扫描电镜(SEM),探讨环境胁迫对微生物表面形貌和菌体生长的影响.结果表明,初始pH 2.0,温度30℃,转速150r/min,氧化亚铁硫杆菌培养60h,酶的活性较高,其中最大值为2.8U/mL,金属离子Zn2+、Cu2+、Mg2+会降低微生物ATP酶的活性,当Zn2+浓度小于0.03mol/L,Cu2+浓度小于0.08mol/L,Mg2+浓度小于0.04mol/L时,对微生物的抑制作用不明显.初始pH=2.0,温度30℃,转速150r/min,培养嗜酸氧化亚铁硫杆菌,与对照菌株相比,菌体形状大小没有明显变化,菌体数量显著增加,菌体表面有生物膜形成.

The effect of some factors on the growth and ATP-ase activity of Acidithiobacillus ferrooxidans, such as solution temperature, pH, dissolved oxygen and the concentration of metal ions is studied. The influence of environmental condition on microbial surface and the growth of Acidithiobacillus ferrooxidans is investigated by the scanning electron microscope{SEM} of per-cultivated and post-cultivated Acidithiobacillus ferrooxidans . The results show that under the condition of initial pH 2.0, temperature 30 ℃, speed 150 r/min, cultivated time 60 h, the ATP-ase activity becomes higher, and the largest ATP-ase activ- ity is 2.8 U/mL,Zn^2＋ ,Cu^2＋ and Mg^2＋ can reduce ATP-ase activity, however, when the concentration of Zn^2＋ , Cu^2＋ and Mg^2＋ is less than 0.03, 0.08 and 0.04 mol/L respectively, the inhibitory effect is not obvi- ous. The scanning electron microscope（SEM） of per-cultivated and post-cultivated Acidithiobacillus ferrooxidans show that the number of Acidithiobacillus ferrooxidans increase remarkably, biofilms grew in the surface of Acidithiobacillus ferrooxidans, appreciable change is not found in the shape size of Acidithiobacillus ferrooxidans.