秋水仙碱对大鼠胆管结扎所致胆汁淤积模型肝损伤的影响

Effects of colchicine on cholestatic liver injury in rats induced by bile duct ligation

考察秋水仙碱对大鼠胆管结扎所致胆汁淤积模型肝损伤的干预作用。采用胆总管结扎建立大鼠胆汁淤积模型,模型动物分别灌胃秋水仙碱0.12和0.24 mg/kg,连续7 d。通过血清生化指标、肝脏髓过氧化物酶(MPO)活力及肝脏组织病理学检查来评价秋水仙碱的作用。结果显示,秋水仙碱可显著降低模型动物血清ALP、γ-GTP、TBA水平。秋水仙碱高、低剂量组对肝脏MPO活力均有显著抑制作用,同时可明显改善肝脏的组织病理学改变。实验结果表明,小剂量的秋水仙碱(≤0.24 mg/kg)对于大鼠胆管结扎所致的胆管损伤具有明显的改善作用,而抑制炎性细胞活化和浸润可能是其重要的作用机制。

To investigate the effects of colchicine on the cholestasis in rats induced by bile duct ligation（ BDL）,colchicine（ 0. 12 and 0. 24 mg / kg） was administered intragastrically for 7 consecutive days after ligation of common bile duct. The effect of colchicines on the liver injury was evaluated by serum biochemistry（ ALT, AST, ALP,γ-GTP,TBIL,DBIL,ALB and TBA）,liver myeloperoxidase（ MPO） activity as well as histological examination. Both doses of colchicine significantly decreased serum ALP,γ-GTP and TBA levels,inhibited liver MPO activity and alleviate liver histological changes,such as inflammatory cell infiltration and interlobular bile duct hyperplasia.Data suggested that lower dose of colchicines（ ≤0. 24 mg / kg） exerted protection of bile duct injury induced by BDL in rats involving primary mechanism on inhibiting activation and infiltration of inflammatory cells.