摘要
利用固氮菌、酵母菌和高产纤维素酶的细菌,采用混菌固态发酵的方式,以制备的植物栽培基质中蛋白质的含量为衡量指标,对棉秆发酵培养基进行优化。结果表明,在最优培养基为棉秆4.7 g,椰树果壳粉0.3 g,蛋白胨0.8 g,酵母粉0.1 g,尿素1.72 g,磷酸氢二钾0.304 g,水35 m L时,制备的植物栽培基质蛋白质含量达到29.08%,与发酵前(19.80%)相比提高了9.28个百分点。
Using azotobacter,yeasts and bacteria with high-yield cellulose,mixed solid-state fermentation,response surface methodology,the fermentation medium of preparing plant cultivation matrix was optimized.The results showed that the optimal fermentation medium was 4.7 g cotton stalk,coconut shell powder 0.3 g,peptone 0.8 g,yeast powder 0.1 g,urea 1.72 g,potassium hydrogen phosphate 0.304 g,and water 35 mL.Under the optimal medium,content of protein reached 29.08%,increasing by 9.28 compared with the content of protein (19.80%) before the fermentation.
出处
《湖北农业科学》
北大核心
2014年第21期5241-5244,5248,共5页
Hubei Agricultural Sciences
基金
武汉市科技局攻关项目(2013020501010184)
关键词
棉秆
复合微生物
蛋白质
发酵
优化
cotton stalk
Compound microbial
protein
fermentation
optimization