复合微生物发酵棉秆制备植物栽培基质的培养基优化

Optimizing Medium of Preparing Plant Cultivation Matrix with Compound Microbial Fermentation of Cotton Stalk

利用固氮菌、酵母菌和高产纤维素酶的细菌,采用混菌固态发酵的方式,以制备的植物栽培基质中蛋白质的含量为衡量指标,对棉秆发酵培养基进行优化。结果表明,在最优培养基为棉秆4.7 g,椰树果壳粉0.3 g,蛋白胨0.8 g,酵母粉0.1 g,尿素1.72 g,磷酸氢二钾0.304 g,水35 m L时,制备的植物栽培基质蛋白质含量达到29.08%,与发酵前(19.80%)相比提高了9.28个百分点。

Using azotobacter,yeasts and bacteria with high-yield cellulose,mixed solid-state fermentation,response surface methodology,the fermentation medium of preparing plant cultivation matrix was optimized.The results showed that the optimal fermentation medium was 4.7 g cotton stalk,coconut shell powder 0.3 g,peptone 0.8 g,yeast powder 0.1 g,urea 1.72 g,potassium hydrogen phosphate 0.304 g,and water 35 mL.Under the optimal medium,content of protein reached 29.08％,increasing by 9.28 compared with the content of protein （19.80％） before the fermentation.