SPE-HPLC法对豆腐柴茎中山奈素和芦丁含量的测定

Determing Kaempferol and Rutin in the Stem of Premna microphylla turca.with SPE-HPLC

采用SPE-HPLC法分析了豆腐柴(Premna microphylla turca.)茎中的山奈素和芦丁,以70%(V/V)甲醇为流动相,C18(4.6 mm×250 mm,5μm)色谱柱分离,在360 nm下检测豆腐柴茎中的山奈素和芦丁含量,同时分析了该方法的精确度、重复性、稳定性和回收率。结果表明,该方法具有较好的重现性,精密度和稳定性(RSD均小于3%)。该方法对评价豆腐柴药用价值和指导开发豆腐柴具有参考价值。

The kaempferol and rutin in the stem of Premna microphyUa turca, were determined with SPE-HPLC. The sample was separated by Cls column （4.6 mm×250 mm, 5μm） with 70%（V/V） methanol at the flow rate of 0.8 mL/min. The wavelength of the UV detector was 360 nm. Its accuracy, repeatability, stability, and recovery was analyzed. The results showed that the method was accurate, stable and repeatable reproducibility. It will provide a reference for further developing and applying medicinal value of Premna microphylla turca.