Preparation of Antibacterial Electrospun PVA / Regenerated Silk Fibroin Nanofibrous Composite Containing Ciprofloxacin Hydrochloride as a Wound Dressing

Electrospinning technique was used for the fabrication of poly( vinyl alcohol)( PVA) / regenerated silk fibroin( SF)composite nanofibers,loaded with ciprofloxacin HCl( CipH Cl) as a wound dressing.Electrospun PVA / SF / CipH Cl composite nanofibers were stabilized against dissolving in water by heating in an oven at155 ℃ for 5 min.Incorporation of CipH Cl into electrospun nanofibers was confirmed by SEM and FT-IR spectra.Further the mechanical properties test illustrated that the addition of CipH Cl enhanced the mechanical properties of PVA and PVA / SF nanofibers.The antibacterial activities against Escherichia coli( E.coli)( gram-negative) and Staphylococcus aureus( S.aureus)( gram-positive) organisms were evaluated by disk diffusion method;and results suggested that electrospun PVA / CipH Cl and PVA / SF /CipH Cl composite nanofibers showed a remarkable antibacterial activity.

Electrospinning technique was used for the fabrication of poly （ vinyl alcohol ） （ PVA ） / regenerated silk fibrnin （ SF ） composite nanofibers, loaded with ciprofloxacin HCI （CipHCI） as a wound dressing. Electrospun PVA/SF/CipHCI composite nanofibers were stabilized against dissolving in water by heating in an oven at 155℃ for 5 min. Incorporation of CipHCi into electrospun nanofibers was confirmed by SEM and FT.IR spectra. Further the mechanical properties test illustrated that the addition of CipHCI enhanced the mechanical properties of PVA and PVA/SF nanofibers. The antibacterial activities against Escherichia coU （E. coli ） （ gram-negative ） and Staphylococcus aureus （ S. aureus ） （gram-positive） organisms were evaluated by disk diffusion method; and results suggested that electrospun PVA/CipHCI and PVA/SF/ CipHCI composite nanofibers showed a remarkable antibacterial activity.