IMP3与p16在宫颈癌中的表达及其临床意义

Expression and clinical significance of IMP3 and p16 in cervical carcinoma

目的 检测胰岛素样生长因子ⅡmRNA结合蛋白3（IMP3）与p16在宫颈癌的表达,探讨其临床意义.方法 采用免疫组织化学Envision二步法检测67例宫颈癌、58例宫颈上皮内瘤变及15例正常宫颈组织中IMP3及p16的表达情况.结果 IMP3在宫颈癌组织细胞质中表达,阳性表达率85.07％（57/67）,显著高于宫颈上皮内瘤变12.07％（7/58; χ^2 =66.32,P＜0.05）及正常宫颈组织的阳性表达率0（0/15;χ^2 =44.38,P＜0.05）.IMP3在宫颈腺癌及宫颈鳞状细胞癌中均呈高表达,两者差异无统计学意义（χ^2=0.35,P＞0.05）.p16在宫颈癌组织细胞质和细胞核均有表达,阳性表达率为83.58％（56/67）,显著高于宫颈上皮内瘤变41.37％（24/58;χ^2 =24.85,P＜0.05）及正常宫颈组织的阳性表达率0（0/15;χ^2 =42.61,P＜0.05）.结论 IMP3与p16在宫颈癌中高表达,可能作为预测宫颈癌转移潜能及预测宫颈上皮内瘤变进展为宫颈浸润癌的指标.

Objective To detect the expression of insulin-like growth factor Ⅱ mRNA-binding protein 3 （IMP3） and p16 in cervical carcinoma and investigate their clinical significance.Methods Immunohistochemical Envision method was used to detect the expression of IMP3 and p16 proteins in 67 cases of carcinoma of the uterine cervix,58 cases of cervical intraepithelial neoplasia （CIN） and 15 cases of normal cervical epithelium.Results IMP3 was mainly dedected in the cytoplasm,whose positive expression rate was 85.07% （57/67） in cervical carcinoma,12.07% （7/58） in CIN （χ^2 =66.32,P 〈 0.05） and 0 （0/15） in cervical epithelium （χ^2 =44.38,P 〈 0.05）.No significance variations of the expression of IMP3 were observed in cervical adenocarcinoma and squamous cell carcinoma （χ^2 =0.35,P 〉 0.05）.p16 was found both in nucleus and cytoplasm of cervical carcinoma,whose expression rate was 83.58% （56/67）,higher than 41.37% （24/58） in CIN （χ^2 =24.85,P 〈 0.05） and 0 （0/15） in cervical epithelium （χ^2 =42.61,P 〈 0.05）.Conclusion Expression of IMP3 and p16 is high in cervical carcinoma,maybe can be used as a potential biomarker of metastasis and progression.