脑脊液降钙素原及乳酸脱氢酶动态测定在小儿化脓性脑膜炎中诊疗的应用

Dynamic research of procalcitonin and lactate dehydrogenase of Cerebrospinal fluid in purulent meningitis children

目的研究化脓性脑膜炎患儿的脑脊液中降钙素原及乳酸脱氢酶浓度的动态变化。方法选取化脓性脑膜炎患者作为观察组,健康者为对照组,每组患者各20例,脑脊液乳酸脱氢酶(LDH)检测使用强生VITROS 350全自动干化学生化分析仪进行。降钙素原(PCT)检测使用梅里埃Mini VIDAS全自动免疫分析仪进行检测。结果与对照组患儿进行比较,观察组脑脊液降钙素原及乳酸脱氢酶水平均显著升高(t=2.869,P=0.007;t=2.284,P=0.018)。经有效治疗72 h后,患儿脑脊液降钙素原及乳酸脱氢酶水平显著下降。化脓性脑膜炎患儿PCT与LDH水平呈正相关关系(r=0.453,P=0.0455)。结论脑脊液中降钙素原及乳酸脱氢酶的浓度联合检测有助于化脓性脑膜炎的诊断,也助于病情的动态观察及疗效评估。

Objective To study the quantificantion of procalcitonin（PCT） and lactate dehydrogenase（LDH） of cerebrospinal fluid in children with purulent meningitis. Methods Total of 20 cases with purulent meningitis were collected as the observation group and 20 cases of pediatric patients as control group. The concentration of LDH was analyzed by the VITROS 350 dry biochemical analyzer. The concentration of PCT was analyzed by the Bio Merieux mini VIDAS immunoassay analyzer. Results The procalcitonin and lactate dehydrogenase of CSF in children with purulent meningitis were significantly increased（t = 2.869, P = 0.007; t = 2.284, P = 0.018）. After 72 hours effective treatment, the procalcitonin and lactate dehydrogenase levels of CSF in children with purulent meningitis were significantly decreased. The procalcitonin of CSF in children with purulent meningitis was correlation with lactate dehydrogenase（r = 0.453, P = 0.045）. ConclusionsThe dynamic measurement of PCT and LDH is useful for the diagnosis of purulent meningitis, also helps to dynamic observation of the disease and treatment evaluation.