胶原—壳聚糖人工支架的理化性质和相容性分析

Collagen-Chitosan compound scaffold Construction and the physicochemical property/biocompatibility analysis

目的体外构建胶原—壳聚糖复合支架材料,分析其物理化学性质及生物相容性,探讨其应用于组织工程支架材料的可行性。方法利用冷冻干燥的方法构建三维多孔的胶原支架材料,通过甲醛交联以及添加壳聚糖的方法改善其物理化学性能。通过体外降解实验以及电镜扫描的方法检测材料的各项物理化学指标;通过细胞接种的方法研究材料的生物相容性。结果胶原—壳聚糖复合材料通过冷冻干燥的方法,能够获得稳定的三维多孔结构,电镜显示孔隙贯通,体外降解速度降低,并且能够支持细胞生长。理化性质分析显示该结构适合细胞生长,具有良好的生物相容性。结论本课题体外构建胶原—壳聚糖复合支架材料,满足组织工程生物材料的理化以及生物相容性要求,为其应用于组织工程支架材料提供重要的依据。

Objective We build up the Collagen-Chitosan compound scaffold in vitro, and study the physical, chemi- cal and biological properties, to analyze the feasibility in tissue engineering. Methods The three-dimensional porous scaffold was obtained by freezing-drying method, and optimized by using formaldehyde and Chitosan. We used hydrolysis in vitro and SEM scanning to investigate its physical and chemical proporties. The biocompatibility of scaffold was analyzed in MEF cells. Results Collagen-Chitosan compound scaffold we obtained by freezing-drying method was a kind of stable 3D vesicular structure. The scaffold degenerated in decreased velocity in vitro. The physical and chemical properties showed that it was suitable for the cells grow in it, which suggested that it has a good biocompatibility. Conclusion This kind of Collagen-Chitosan compound scaffold is constructed. It＇s qualified by the physical and chemical properties, and biocompatibility which the biomaterials require. The evidence are important for its application in tissue engineering.