TRAIL及其受体在多发性肌炎/皮肌炎患者外周血CD3^+T淋巴细胞中的表达

The expression of tumor necrosis factor-related apoptosis-inducing ligand on CD3^+T lymphocytes in patients with polymyositis/dermatomyositis

目的:通过检测多发性肌炎/皮肌炎(PM/DM)患者外周血CD3+T淋巴细胞表面肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体DR4、DR5的表达,探讨TRAIL及其受体在PM/DM患者T淋巴细胞凋亡中的作用。方法:采用流式细胞术对44例PM/DM患者和20例健康人的外周血CD3+T淋巴细胞表面TRAIL及其受体DR4、DR5的表达进行检测。用流式细胞术检测外周血单个核细胞的凋亡率。结果:PM/DM组的m TRAIL、DR4、DR5的阳性表达率均显著高于健康对照组(均P<0.05)。不同浓度TRAIL(0,5,10,50,100ng/ml)刺激淋巴细胞24h后,检测其凋亡率,PM/DM组淋巴细胞凋亡率(%)分别为25.63±8.79,33.04±12.31,42.42±10.72,80.74±14.52,69.9±16.99,均显著高于对照组的凋亡率(%):18.72±12.48,23.23±17.00,24.69±9.15,26.64±13.28,20.32±11.07。结论:TRAIL及其受体DR4、DR5在多发性肌炎/皮肌炎患者外周血CD3+T淋巴细胞表面的表达阳性率增高,提示TRAIL介导的细胞凋亡可能在多发性肌炎/皮肌炎的发病机制中发挥一定的作用。

Objective：To detect the expression of tumor necrosis factor-related apoptosis-inducing ligand（m TRAIL）and its death receptors DR4,DR5 on the surface of CD3＋T lymphocytes in patients with polymyositis/dermatomyositis（PM/DM）,and to investigate the effect of TRAIL on the apoptosis of CD3＋T lymphocytes.Methods：The flow cytometry was employed to detect the expression of TRAIL and its death receptors DR4,DR5 on CD3＋T lymphocytes in 44 patients with PM/DM and 20 healthy subjects（control group）.Results：The positive expression rate of m TRAIL,DR4 and DR5 on peripheral blood CD3＋T lymphocytes in PM/DM group were 28.39±9.16%,27.09±10.34%,29.06±9.54%,respectively.They were 19.87±8.40%,19.72±8.59%,19.22±9.27% in control group,respectively（P〈0.05）.The apoptosis ratio of T lymphocytes cultured by different concentrationof TRAIL（0,5,10,50,100ng/ml）in PM/DM group was 25.63±8.79%,33.04±12.31%,42.42±10.72%,80.74±14.52%,69.9±16.99%,respectively.And in control group,the ratio（%）was 18.72±12.48%,23.23±17.00%,24.69±9.15%,26.64±13.28%,20.32±11.07%,respectively.The apoptosis ratio of T lymphocytes in patient group was significantly higher than in control group.Conclusion：The increased expression of m TRAIL and its death receptors DR4,DR5 on the surface of peripheral blood CD3＋T lymphocytes in patients with PM/DM suggested that they may have played a role in the pathogenesis of PM/DM.