玉米单倍体诱导率的优化研究

Studies on the optimization of maize haploid induced rates

【目的】提高玉米单倍体诱导率.【方法】2011—2012年采用大田试验,分析母本基因型材料、种质和世代的选择、生态环境、授粉时期、果穗部位等5个处理因素对玉米单倍体诱导率的影响.【结果和结论】不同材料同一因素下以及同一材料不同因素下单倍体诱导率均存在差异.母本基因型间单倍体的诱导率差异显著,尚未发现不同杂交模式与诱导率之间存在明显的相关性;5大种质类群S1代为单倍体最佳诱导世代;三亚与长春相比更有利于提高单倍体的诱导率;早期授粉的玉米单倍体诱导率高于晚期授粉;果穗底部的单倍体诱导率低于顶部.

【Objective 】To optimize the technical measures to improve maize haploid induced rates.【Method】Effects of five factors,including parent genotypes,selection of germplasm and generations,environments,pollination period,parts of maize ear on the rates of maize haploid were conducted in the field from 2011 to 2012.【Result and conclusion】The haploid induced rates were different among different materials with the same factor and different among different factors with the same materials. The haploid induced rates in female parent genotypes were significantly different. There was no significant correlation between the crossing pattern and the induced rate. The optimal induced haploid generation of five germplasms was S1. Compared to Changchun,Sanya was more conducive to improve the induced rate of haploid. The haploid induced rate of early pollination maize was higher than that of the late ones. The haploid induced rate at the top of the maize ear was higher than that at the bottom.