枸杞多糖对四氯化碳致建鲤急性肝损伤的保护作用

Protective effects of Lycium barbarum polysaccharides against carbon tertrachloride(CCl_4)-induced liver accurate injury in Carprinus carpoivar Jian

研究枸杞多糖(LBP)对四氯化碳诱导建鲤肝组织急性损伤的保护作用。将建鲤随机分为6个组,即空白对照组(CK)、模型组(CCl4)、枸杞多糖药物对照组(LBP CK)、处理组(LBP 0.1 g·kg-1、0.5 g·kg-1、1.0g·kg-1),连续饲喂60 d后,模型组及处理组按体重0.05 m L·10g-1体内注射30%CCl4-植物油混合液,72 h后收集肝组织及血液,检测谷丙转氨酶(GPT)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、丙二醛(MDA)、总蛋白(TP)、白蛋白(Alb)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、总抗氧化能力(T-AOC)等一系列生化指标。研究结果表明:腹腔注射30%CCl4-植物油可诱导建鲤急性肝组织损伤。1.0 g·kg-1的枸杞多糖能有效降低建鲤血清中GPT、GOT、LDH活性及肝组织中MDA含量,显著提高肝组织中T-AOC、CAT、GSH-PX、SOD、GSH及TP含量(P<0.05);0.5 g·kg-1枸杞多糖对血清中GPT的降低及肝组织中SOD、T-AOC和Alb的提高均有显著作用(P<0.05);0.1 g·kg-1枸杞多糖对血清及肝组织中生化指标无显著效果。综上结果,认为枸杞多糖对四氯化碳致建鲤肝损伤具有一定保护作用,可以应用于鱼类肝损伤的防治。

The aiming of this study was evaluating if the Chinese herbal medicine Lycium barbarum polysaccharides had hepatoprotective effects against CC14-induced liver accurate injury in Cyprinnus var. Jian. The carps were ran- domly divided into 6 treatment groups, which named blank the control group （ Ctrl）, model group （ CC14 ）, Lycium barbarum polysaccharides drug the control group （ LBP Ctrl）, experimental group （ LBP 0. 1 g. kg- 1,0. 5 g. kg- 1, 1.0 g. kg - 1 ）. After fed for 60 days, the model group and Lycium barbarum polysaccharides drug control group were injected （ i. p） with 30% （V/V） of CC14 in arachis oil at a volume of 0. 05mL 10g-1 of body weight, after 72 h , the liver samples and blood were collected for detccting glutamic pyruvic transaminase （ GPT） , glutamate oxalate transaminase （GOT） , lactatedehydrogenase （LDH） , superoxide dismutase （SOD） , malondialdehyde （MDA） , to- talprotein （TP）, lbumin （Mb）, catalase （CAT）, glutathione peroxidase （GSH-Px）, Glutathione （GSH） and total antioxidant capacity （T-AOC）. The results showed that CCl4 could indnce the liver injury of Jian Carp; the 1.0 g kg-1 LBP could effectively reduce the value of GPT, GOT, LDH and MDA and improve the value of T-AOC, CAT, GSH-Px, SOD, GSH and TP （ P 〈 0. 05 ） ; 0. 5 g kg-1LBP could effectively reduce the value of GPT and improve the value of T-AOC, SOD. But 0. 1 g-kg- i LBP had no obvious changes on these indexes. From the above results, it can be indicated that LBP had hepatoprotective effects against carbon tertrachloride （ CC14 ） -induced liver accurate injury., and could be used for fish liver disease prevention and control.