多粘类芽孢杆菌的保藏及复壮方法研究

Preservation method and rejuvenation method of Paenibacillus polymyxa

研究了保护剂、保藏及复壮方法对多粘类芽孢杆菌C12菌种存活率和产抗生素能力的影响。结果表明,以20%脱脂奶粉为保护剂,冻藏的存活率是冻干的17.3倍。冻藏中,20%脱脂奶粉添加海藻糖和二甲亚砜后,菌种的抗生素相对效价平均值比不添加的分别提高4.5%和4.1%。冻干中,20%脱脂奶粉添加海藻糖,菌种的抗生素相对效价平均值提高6%,存活率提高5倍。冻藏6个月的菌种经过3次循环分离纯化后,菌株产抗生素能力最高恢复至93%,平均恢复至80%;经抗性平板1次分离纯化复壮,菌株产抗生素能力最高恢复至81%,平均恢复至45%。

The effects of protective agent, preservation method and rejuvenation method on Paenibacillus polymyxa C12＇s survival rate and production ability were studied. The results were as following, using 20% skimmed milk pow- der as protective agent, the survival rate of the strains in-80℃ preservation was 17. 3 times higher than the freeze-dr- ying preservation; In the-80℃ preservation, adding thehalose and dimethyl sulfoxde into 20% skimmed milk, the av- erage relative antibiotic titer increased by 4. 5% and 4. 1% respectively; In the freeze-drying preservation adding thchalose into 20% skimmed milk the average antibiotic production increased by 6.0% , and the survival rate in- creased by 5 times ; The strains preserved in-80℃ for six months, then separated and purified for three times, the highest antibiotic production capacity returned to 93%, and the average antibiotic production capacity returned to 80% ; Using resistance plate to separate and purify for one time, the highest antibiotic production capacity returned to 81%, and the average antibiotic production capacity returned to 45%.