Nesfatin-1在非酒精性脂肪肝病患者血清中的表达及意义

Expression and significance of Nesfatin-1 in serum of patients with nonalcoholic fatty liver disease

目的研究Nesfatin-1在非酒精性脂肪肝病患者血清中的表达及意义。方法采用酶联免疫吸附ELISA法检测54例非酒精性脂肪肝病患者及50例正常对照血清Nesfatin-1的表达水平;全自动生化分析仪检测两组空腹血糖(FPG),甘油三酯(TG),总胆固醇(TC),高密度脂蛋白(HDL),低密度脂蛋白(LDL)及丙氨酸氨基转移酶(ALT)水平,计算体重指数。结果与正常对照组相比,NAFLD组Nesfatin-1及HDL的表达水平显著降低,差异有统计学意义(P<0.05),BMI及FPG,TG,TC,LDL,ALT的水平显著增高,差异有统计学意义(P<0.05);随着NAFLD患者病变程度的增加,血清Nesfatin-1的表达水平逐渐下降,差异有统计学意义(P<0.05);血清Nesfatin-1的表达水平与NAFLD患者FPG,TG及体重指数呈负相关(P<0.05),与TC,HDL,LDL,ALT无显著相关性(P>0.05)。结论 Nesfatin-1可能在NAFLD的发病过程中起到重要作用,检测血清Nesfatin-1水平可作为NAFLD辅助诊断及判断疾病严重程度的一个参考指标。

Objective To explore the expression of Nesfatin-1 and its significance in serum of patients with nonalcoholic fatty liver disease（NAFLD）. Methods Serum Nesfatin-1 levels in 54 NAFLD patients and 50 normal controls were detected by enzyme-linked immunosorbent assay. Fasting plasma glucose （FPG）, triglyceride （ TG）, total cholesterol （ TC ）, high density lipoprotein （ HDL）, low density lipoprotein （LDL）and alanine aminotransferase （ALT）were detected by automatic biochemical analyzer, and the body mass index （BMI） was calculated. Results Nesfatin-1 and HDL levels in NAFLD patients were significantly lower than those in controls （P 〈 0.05 ）. BMI, FPG, TG, TC, LDL and ALT levels in NAFLD patients were higher than those in controls（ P 〈 0.05 ）. Nesfatin-1 levels in NAFLD significantly decreased with the progression of disease（ P 〈0.05）. Nesfatin-1 level was negatively correlated with FPG,TG and BMI （ P 〈 0.05 ）, but not correlated with TC, HDL, LDL and ALT（ P 〉 0. 05 ）. Conclusion Nesfatin-1 may play an important role in the pathogenesis of NAFLD, and it could be used as an auxiliary marker for the diagnosis and prognosis of NAFLD.