比较人AB型血清与胎牛血清在结核杆菌裂解物刺激人外周血单个核细胞中的作用

Comparsion of Mycobacterium tuberculosis Whole Cell Lysate effects on proliferation of human peripheral blood mononucelear cells in human AB type serum and fetal bovine serum

目的观察添加人AB型血清或胎牛血清(FBS)培养基培养的人外周血单个核细胞(PBMC)在结核杆菌(M.tb)全菌裂解物(WCL)的刺激下增殖差异。方法健康成人PBMC 1×109·L-1,分别加入WCL 20 mg·L-1,或者同时给予人重组白细胞介素-2(rh IL-2)50 U·m L-1,植物血凝素(PHA)5 mg·L-1,同时设置空白对照组。分别在含10%人AB型血清的RPMI1640培养液和10%FBS的RPMIl640培养液中培养6 d后,用倒置显微镜观察培养结果,运用流式细胞术检测淋巴细胞增殖情况。结果添加人AB型血清培养基培养条件下,M.tb WCL对淋巴细胞增殖效果较同样条件下添加FBS培养的增殖效果明显,差异有统计学意义(P<0.05)。在WCL和rh IL-2混合组中同样是添加人AB型血清培养增殖效果明显,差异有统计学意义(P<0.05)。结论当以M.tb WCL为抗原的刺激人PBMC时,淋巴细胞在添加人AB型血清培养液中比在添加FBS的培养液中增殖效果更加明显。

Objective To observe the proliferation difference of human peripheral blood mononucelear cells （ PBMCs） which were stim-ulated with Mycobacterium tuberculosis （ M.tb） Whole Cell Lysate （ WCL） in human AB serum cell culture medium and fatal bovine serum （ FBS） cell culture medium.Methods PBMCs from healthy adults were stimulated by WCL or WCL with recombinant human interleukin 2 （ rhIL-2） or phytohemagglutinin （ PHA） or none as control in four groups.Each of the groups was cultured with RPMI-1640 medium containing 10%AB serum or 10%FBS for six days respectively.The proliferation of lymphocytes was carefully investiga-ted under the inverted microscope and flow cytometry.Results The proliferation of lymphocytes stimulated by WCL in medium with 10%AB serum is better than that in medium with 10%FBS （P〈0.05）.Similar results were obtained when PBMCs were stimulated by WCL and rhIL-2 （P〈0.05） .Conclusion When human PBMCs were stimulated with M.tb WCL,the proliferation of lymphocytes was better in the culture medium supplemented with human AB serum than in that with FBS serum.