地黄饮子对转基因果蝇AD模型mTOR信号通路中4E结合蛋白和p70核糖体S6蛋白激酶表达的影响

Effects of Rehmanniae Decoction on the expression of 4E-BP and p70S6K in the m TOR signaling pathway of AD transgenic drosophila model

目的:研究地黄饮子对转tau基因果蝇m TOR信号通路中4E结合蛋白(4E-BP)和p70核糖体S6蛋白激酶(p70S6K)表达的影响,探讨其防治AD的疗效和作用机制。方法:将果蝇分为3组,即空白组、地黄组、模型组,空白组为正常CS果蝇喂普通培养基,地黄组为转tau基因果蝇喂2%浓度地黄饮子培养基,模型组为转tau基因果蝇喂普通培养基,均喂养5d。采用荧光定量PCR技术检测果蝇4E-BP和p70S6K的m RNA表达,Western blot方法检测4E-BP和p70S6K蛋白的表达。结果:地黄饮子能够降低4E-BP和p70S6K的m RNA和蛋白的表达,且与模型组比较差异均具有统计学意义(P<0.01)。结论:地黄饮子通过调控m TOR信号通路中4E-BP和p70S6K的m RNA和蛋白的表达,调节了细胞周期,抑制了细胞的凋亡,提高转tau基因果蝇AD模型学习记忆能力。

Objective： Study of the effects of Rehmanniae Decoction（RD） on the expression of 4E-BP and p70S6 K in the m TOR signaling pathway of AD transgenic drosophila model, to investigate the mechanisms of Rehmanniae Decoction in the treatment and prevention of AD. Methods： The drosophila were divided into three groups, included blank group, RD group and model group. The blank group was normal CS drosophila which was fed normal culture medium, the RD group was tau transgenic drosophila which was fed 2% RD culture medium, while the model group was tau transgenic drosophila which was fed normal culture medium, and every group was fed for 5 days. RT-PCR was used to detect the m RNA expression of 4E-BP and p70S6 K genes, the protein expression of 4E-BP and p70S6 K were detected by Western blot method. Results： RD could reduce the m RNA expression and protein expression of 4E-BP and p70S6 K, there were statistical significant differences compared with model group（P0.01）. Conclusion： RD could effectively enhance the capability of learning and memory of AD transgenic drosophila model through regulating the expression of 4E-BP and p70S6 K in m TOR signaling pathway, regulating the cell cycle and inhibiting the cells apoptosis.